FastQCFastQC Report
Sat 18 Jun 2016
SRR1930900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729152
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.178353320008883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.1578808572063069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.051.0283781
GTATCAA36150.038.350611
TCAACGC44950.030.7298554
ATCAACG45350.030.250343
CAACGCA46250.029.8574585
TATCAAC48300.028.6009652
AACGCAG48750.028.423656
TAACGCA1606.287155E-826.7005214
ACGCAGA56400.024.3158997
GTACATG46450.023.815951
TACATGG46200.023.7358552
CGCAGAG57650.023.7063548
ACATGGG46650.023.5055333
TGGTATC10800.022.4171962
GTGGTAT11100.021.8144841
TATACCG1553.937279E-521.4307795
CTAACGC1150.003738155420.638683
TCTAACG1404.8726355E-420.3450182
GCAGAGT64750.020.227449
CATGGGG31650.019.796914
Produced by FastQC (version 0.11.5)