##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930900_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1729152 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.211482275705084 31.0 31.0 33.0 30.0 34.0 2 31.32220938355911 31.0 31.0 34.0 30.0 34.0 3 31.401962927492782 31.0 31.0 34.0 28.0 34.0 4 35.272626697942115 37.0 35.0 37.0 33.0 37.0 5 35.061215555370495 35.0 35.0 37.0 32.0 37.0 6 35.098067145051445 37.0 35.0 37.0 32.0 37.0 7 34.98184543637575 36.0 35.0 37.0 32.0 37.0 8 34.95997113035754 36.0 35.0 37.0 32.0 37.0 9 36.462348596306164 38.0 35.0 39.0 32.0 39.0 10-11 36.38802112249241 38.0 35.0 39.0 32.0 39.0 12-13 36.28526613045007 38.0 35.0 39.0 32.0 39.0 14-15 37.279522274502185 39.0 36.0 41.0 32.0 41.0 16-17 37.24300726598934 39.0 36.0 40.0 32.0 41.0 18-19 37.23249749009919 39.0 36.0 40.0 31.0 41.0 20-21 37.20236451162188 39.0 36.0 40.0 31.0 41.0 22-23 37.066578299652086 39.0 36.0 40.0 31.0 41.0 24-25 36.94109395819454 39.0 36.0 40.0 31.0 41.0 26-27 36.65359754376712 38.5 35.0 40.0 30.0 41.0 28-29 36.45162397522023 38.0 35.0 40.0 30.0 41.0 30-31 36.21030308498038 38.0 35.0 40.0 30.0 41.0 32-33 35.957320698238206 38.0 34.0 40.0 29.0 41.0 34-35 36.14345384327115 38.0 35.0 40.0 30.0 41.0 36-37 36.17805259456659 38.0 35.0 40.0 30.0 41.0 38-39 36.140456131097785 38.0 35.0 40.0 30.0 41.0 40-41 36.03979089172034 38.0 35.0 40.0 29.5 41.0 42-43 35.89311523798949 38.0 35.0 40.0 29.0 41.0 44-45 35.698671082704124 38.0 34.0 40.0 28.0 41.0 46-47 35.466219858057585 38.0 34.0 40.0 27.0 41.0 48-49 35.258036598286324 38.0 34.0 40.0 27.0 41.0 50-51 34.98403032237768 38.0 33.5 40.0 26.0 41.0 52-53 34.76932999528093 38.0 33.0 40.0 26.0 41.0 54-55 34.59269717179288 38.0 33.0 40.0 26.0 41.0 56-57 34.33937849304168 37.0 33.0 40.0 25.0 41.0 58-59 34.099884220704716 37.0 33.0 40.0 25.0 41.0 60-61 33.785463047782955 37.0 32.0 40.0 23.5 41.0 62-63 33.47825321313569 36.0 32.0 39.0 23.0 41.0 64-65 33.16479175919757 36.0 31.5 39.0 23.0 40.5 66-67 32.82764326097417 35.5 31.0 39.0 22.0 40.0 68-69 32.461169405581465 35.0 31.0 38.5 22.0 40.0 70-71 32.05143301456437 35.0 31.0 38.0 21.0 40.0 72-73 31.620128826152936 35.0 30.0 37.0 20.0 39.5 74-75 31.0887961266563 34.0 30.0 37.0 18.0 39.0 76-77 29.73895730392701 32.5 28.5 35.5 17.5 38.0 78-79 30.500423039732773 34.0 29.5 36.0 18.0 38.5 80-81 30.488868532089718 34.0 30.0 35.5 18.0 37.5 82-83 30.20060989432971 34.0 29.5 35.0 17.5 37.0 84-85 29.865161362332522 34.0 29.0 35.0 14.0 36.5 86-87 29.50937627229995 34.0 29.0 35.0 9.5 36.0 88-89 29.152754645051445 34.0 29.0 35.0 7.0 36.0 90-91 28.794477292915836 33.5 29.0 35.0 2.0 35.5 92-93 28.413053623972907 33.0 29.0 35.0 2.0 35.0 94-95 27.97056649733511 33.0 27.0 35.0 2.0 35.0 96-97 27.48633145032941 33.0 27.0 35.0 2.0 35.0 98-99 26.967811967947295 33.0 26.0 35.0 2.0 35.0 100-101 25.732500381689984 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6055103459683515 1101 2 0.5819343402890311 1101 3 0.5722111368217107 1101 4 0.480906037470092 1101 5 0.5395983571745973 1101 6 0.5684884531748438 1101 7 0.6838954825281363 1101 8 0.45769595861068524 1101 9 0.6632010599405049 1101 10-11 0.6514083383891247 1101 12-13 0.6806087688186082 1101 14-15 0.7767388680474525 1101 16-17 0.826786941988118 1101 18-19 0.878987360374289 1101 20-21 0.8137105283824795 1101 22-23 0.8470669712951775 1101 24-25 0.920857156331877 1101 26-27 1.0131275483186784 1101 28-29 0.7294088372397383 1101 30-31 0.9277313766021393 1101 32-33 0.8969350796274966 1101 34-35 0.8710000866378849 1101 36-37 0.9967159718707137 1101 38-39 1.0121454620099755 1101 40-41 0.8955509314630916 1101 42-43 1.1275878242044328 1101 44-45 1.1684668274893966 1101 46-47 0.8934647423472342 1101 48-49 0.9029863966665488 1101 50-51 0.9015842373943954 1101 52-53 0.9846711234070042 1101 54-55 0.9199235678347719 1101 56-57 0.9547380757308801 1101 58-59 0.918183295131648 1101 60-61 0.9415216068589913 1101 62-63 0.9076483745503268 1101 64-65 0.8914083071527799 1101 66-67 0.8962134234954746 1101 68-69 0.8282868378924562 1101 70-71 0.8440362043008918 1101 72-73 0.8796385691264987 1101 74-75 1.0092504958882174 1101 76-77 1.0327718968883772 1101 78-79 0.9351246757295506 1101 80-81 0.8901895525546415 1101 82-83 0.9674784558091645 1101 84-85 0.998668682871493 1101 86-87 1.0902913430401888 1101 88-89 0.8045122276362342 1101 90-91 0.7679288911073137 1101 92-93 0.9834005208408847 1101 94-95 0.9643941094563537 1101 96-97 1.0154210545871898 1101 98-99 1.0755054266161146 1101 100-101 1.2888438639641357 1102 1 0.5174118682259312 1102 2 0.5021138633543174 1102 3 0.5718063121150223 1102 4 0.39980464200789356 1102 5 0.5323267460500531 1102 6 0.38248916494030283 1102 7 0.4131986978540425 1102 8 0.5134541606055762 1102 9 0.8255341467272714 1102 10-11 0.6912044869471501 1102 12-13 0.7089385573698195 1102 14-15 0.9168347943246289 1102 16-17 0.774124077021952 1102 18-19 0.7952793994267395 1102 20-21 0.8314071049721434 1102 22-23 1.031296731518374 1102 24-25 0.8045172124822599 1102 26-27 0.9380223554775711 1102 28-29 1.066395910723358 1102 30-31 1.044261740399861 1102 32-33 0.9031751818416751 1102 34-35 0.7170130656615399 1102 36-37 0.9202089640925024 1102 38-39 1.0406224006147013 1102 40-41 0.8879240858481339 1102 42-43 1.0458244155609577 1102 44-45 1.0052309070593353 1102 46-47 1.1893572463160709 1102 48-49 1.1280622891445944 1102 50-51 0.9805046356978337 1102 52-53 0.9653505482642117 1102 54-55 0.7995172262508774 1102 56-57 0.903432135211915 1102 58-59 1.0606942325293502 1102 60-61 0.8645885445756321 1102 62-63 0.9024592283901356 1102 64-65 0.998566606253739 1102 66-67 0.9608477880244024 1102 68-69 0.8901676489420609 1102 70-71 1.0201271728529093 1102 72-73 0.988891973835166 1102 74-75 1.125893491059486 1102 76-77 1.1097415846255956 1102 78-79 1.0644799445765862 1102 80-81 1.091031549050264 1102 82-83 1.0904931455332267 1102 84-85 1.121128318704283 1102 86-87 1.3116032408674876 1102 88-89 1.3409137152130732 1102 90-91 1.3878607223864883 1102 92-93 1.4162005918877831 1102 94-95 1.293304298009378 1102 96-97 1.3919284513082673 1102 98-99 1.3234221513541122 1102 100-101 1.7025777933308142 1103 1 0.43605504097055103 1103 2 0.48495059980292154 1103 3 0.44751389530216557 1103 4 0.1292319535960189 1103 5 0.2940866470814427 1103 6 0.4045934798021449 1103 7 0.5471232933237147 1103 8 0.4718512849721037 1103 9 0.6001976958143871 1103 10-11 0.5611177803354295 1103 12-13 0.485177365331964 1103 14-15 0.6081403598971065 1103 16-17 0.6938962387647791 1103 18-19 0.5554138322116344 1103 20-21 0.5732826511795182 1103 22-23 0.7384419902779058 1103 24-25 0.676832855861619 1103 26-27 0.7494728512830733 1103 28-29 0.7349601601109086 1103 30-31 0.6631246473203447 1103 32-33 0.709866695463333 1103 34-35 0.6382080936183812 1103 36-37 0.7777131637134147 1103 38-39 0.6286958943014653 1103 40-41 0.7649661475058807 1103 42-43 0.6869226950317753 1103 44-45 0.862386047279827 1103 46-47 0.7018787898259404 1103 48-49 0.5623377406439403 1103 50-51 0.571988035485056 1103 52-53 0.6172638942680564 1103 54-55 0.8650358040391524 1103 56-57 0.9680057180884916 1103 58-59 0.794953193538646 1103 60-61 0.7166508824779569 1103 62-63 0.6450300059584535 1103 64-65 0.6823051543462952 1103 66-67 0.47696435461454456 1103 68-69 0.5395475872663482 1103 70-71 0.5061007919187404 1103 72-73 0.6062956007163365 1103 74-75 0.6409266260363999 1103 76-77 0.8818141376486146 1103 78-79 1.0425904231601244 1103 80-81 0.9953316849524363 1103 82-83 0.876213343624439 1103 84-85 0.8712778239254142 1103 86-87 1.0644648542149007 1103 88-89 0.8944720357240357 1103 90-91 0.8917191441503434 1103 92-93 1.0160546799251833 1103 94-95 0.9739826410552048 1103 96-97 0.8410392481518478 1103 98-99 0.7983089637103014 1103 100-101 1.0246952941681684 1104 1 0.4548175365007303 1104 2 0.5377808105081279 1104 3 0.48118193783397345 1104 4 0.3204748525713086 1104 5 0.3978854111375725 1104 6 0.43148920610042296 1104 7 0.4871705536153641 1104 8 0.4561205875325882 1104 9 0.6068204036103708 1104 10-11 0.6473384745923099 1104 12-13 0.6250904388360965 1104 14-15 0.651435106336919 1104 16-17 0.6897569695912438 1104 18-19 0.6952517942928083 1104 20-21 0.6137265427929677 1104 22-23 0.6150574234788166 1104 24-25 0.7454317037230496 1104 26-27 0.8189922364814919 1104 28-29 0.8688864384796702 1104 30-31 0.8663904428378686 1104 32-33 0.9071186618773197 1104 34-35 0.7347841801074182 1104 36-37 0.7435539016915911 1104 38-39 0.6442682279841989 1104 40-41 0.8197211460251594 1104 42-43 0.8047557750240557 1104 44-45 0.8845305536702881 1104 46-47 0.8946534088620339 1104 48-49 0.9362912495915765 1104 50-51 0.8714417650052866 1104 52-53 0.7686399846893082 1104 54-55 0.8044674271463137 1104 56-57 0.9862009610931466 1104 58-59 0.8814026013104197 1104 60-61 0.7734762835944693 1104 62-63 0.8295210177784398 1104 64-65 0.9185493190028282 1104 66-67 0.6153667515444639 1104 68-69 0.7446627519707931 1104 70-71 0.7623000187472542 1104 72-73 0.7965867811299816 1104 74-75 1.072454346298727 1104 76-77 1.1710782115938905 1104 78-79 1.266042605600088 1104 80-81 1.2132520416533588 1104 82-83 1.229729479831402 1104 84-85 1.1911280680298582 1104 86-87 1.203505621438918 1104 88-89 1.1070716159602405 1104 90-91 1.060164881446525 1104 92-93 1.037130730164126 1104 94-95 1.1988416650552232 1104 96-97 1.213347064286456 1104 98-99 1.248569995105342 1104 100-101 1.5170869217482235 1105 1 0.20201328997810108 1105 2 0.2829871063803324 1105 3 0.3410007015604357 1105 4 0.16699976903931457 1105 5 0.27508780493931084 1105 6 0.2857707488143646 1105 7 0.20632266866426363 1105 8 0.2523107307571095 1105 9 0.3273689225599341 1105 10-11 0.2796567631718929 1105 12-13 0.4027395880298741 1105 14-15 0.4033801289093333 1105 16-17 0.33878415033946396 1105 18-19 0.3413025610977556 1105 20-21 0.30380582779663 1105 22-23 0.5566181695415509 1105 24-25 0.5800726686583815 1105 26-27 0.4568534654342429 1105 28-29 0.6156746508480424 1105 30-31 0.522466808914487 1105 32-33 0.6167181247853577 1105 34-35 0.6701872278520469 1105 36-37 0.5510010665311924 1105 38-39 0.27758461832264203 1105 40-41 0.4930300923957347 1105 42-43 0.47437368067044616 1105 44-45 0.378707951226275 1105 46-47 0.5092450523775085 1105 48-49 0.4818836463028475 1105 50-51 0.5059743260587268 1105 52-53 0.25321002955256233 1105 54-55 0.4415760468974099 1105 56-57 0.46931682184812473 1105 58-59 0.4705030544010995 1105 60-61 0.5900478817774086 1105 62-63 0.5667511519458373 1105 64-65 0.4387281032837933 1105 66-67 0.369550537186349 1105 68-69 0.476188923765136 1105 70-71 0.43437988917879977 1105 72-73 0.6633699663269823 1105 74-75 0.7839652488666324 1105 76-77 0.7358158014611789 1105 78-79 0.8065862733586151 1105 80-81 0.7745827990257617 1105 82-83 0.9043911283212545 1105 84-85 0.8275978791912344 1105 86-87 0.920661826270635 1105 88-89 0.8076460744101119 1105 90-91 0.6063911006382376 1105 92-93 0.6807198254844558 1105 94-95 0.527315252754434 1105 96-97 0.5459387383051926 1105 98-99 0.7216501377197986 1105 100-101 1.034136335851052 1106 1 0.006169184616162227 1106 2 0.08316099222289708 1106 3 0.09724311130540642 1106 4 0.03652155997149009 1106 5 0.043345243389246946 1106 6 0.1986133453256116 1106 7 0.17816684762867396 1106 8 0.09904508103127796 1106 9 0.1200066051860702 1106 10-11 0.18557813963013814 1106 12-13 0.19633467782489333 1106 14-15 0.23900654345685268 1106 16-17 0.1174937149808386 1106 18-19 0.3350624514300691 1106 20-21 0.19475571371280154 1106 22-23 0.22510515570428424 1106 24-25 0.22800386414655094 1106 26-27 0.4092637164186783 1106 28-29 0.32292340190607405 1106 30-31 0.30870567654044123 1106 32-33 0.4070376170202934 1106 34-35 0.34180260662878226 1106 36-37 0.3183169108435635 1106 38-39 0.3001296883370017 1106 40-41 0.3237927060195531 1106 42-43 0.41055257378125276 1106 44-45 0.3287295719527279 1106 46-47 0.18609971969433303 1106 48-49 0.13607277705764886 1106 50-51 0.27015602756389967 1106 52-53 0.1252061286214925 1106 54-55 0.21604196895238914 1106 56-57 0.1426080944348911 1106 58-59 0.2099344867972306 1106 60-61 -0.04770259663866483 1106 62-63 0.03461475966305727 1106 64-65 0.07539697035772974 1106 66-67 0.05582085836913819 1106 68-69 -0.0399840696474385 1106 70-71 -0.03784409004689593 1106 72-73 -0.08794855297526283 1106 74-75 0.1566361331083428 1106 76-77 0.26374966503899344 1106 78-79 0.3051150864267349 1106 80-81 0.2115586689149893 1106 82-83 0.30375814705308457 1106 84-85 0.28695896372367713 1106 86-87 0.14920440530129397 1106 88-89 0.44924285706200706 1106 90-91 0.5416974019299587 1106 92-93 0.42955196124421846 1106 94-95 0.42291733248431385 1106 96-97 0.545376369409599 1106 98-99 0.7302826152710473 1106 100-101 0.8823292226320483 1107 1 0.15326587618499943 1107 2 0.13149055465619242 1107 3 0.24739380500871277 1107 4 0.12116844720023323 1107 5 0.19584556355999894 1107 6 0.2310055189293081 1107 7 0.2660999675148332 1107 8 0.2398314204122869 1107 9 0.22851432485878576 1107 10-11 0.23489340110293 1107 12-13 0.12596171446665494 1107 14-15 0.12221906569094187 1107 16-17 0.1594577135578561 1107 18-19 0.15372669902878755 1107 20-21 0.30976617262421513 1107 22-23 0.2078966178174113 1107 24-25 0.09859824337102907 1107 26-27 0.22632070681355287 1107 28-29 0.2555946221124046 1107 30-31 0.2217800273053001 1107 32-33 0.3047363719117939 1107 34-35 0.16129944049573197 1107 36-37 0.3618173021633737 1107 38-39 0.447399129816894 1107 40-41 0.28282664411987213 1107 42-43 0.36402066342906636 1107 44-45 0.3308700201917887 1107 46-47 0.20791430525107302 1107 48-49 0.1817520371074508 1107 50-51 0.299380360541484 1107 52-53 0.094626121506586 1107 54-55 0.25483926528821144 1107 56-57 0.30850820115847455 1107 58-59 0.3839310716424862 1107 60-61 0.22100362890384417 1107 62-63 0.12450675539411549 1107 64-65 0.22009793086999707 1107 66-67 0.20338286477255707 1107 68-69 0.3638228318111132 1107 70-71 0.22137227423627337 1107 72-73 0.17445301230399934 1107 74-75 0.48478953047582607 1107 76-77 0.525641376173823 1107 78-79 0.6361749227839013 1107 80-81 0.6618705863820828 1107 82-83 0.7563129674017155 1107 84-85 0.7951685688464103 1107 86-87 0.6640836653510966 1107 88-89 0.6929555571687338 1107 90-91 0.48394713512099585 1107 92-93 0.6373988484729622 1107 94-95 0.6741984424096046 1107 96-97 0.7040565543971447 1107 98-99 0.5472758561106055 1107 100-101 0.7444402151613971 1108 1 0.12200266092614243 1108 2 0.25858374266709205 1108 3 0.187831952147679 1108 4 0.09330432367616481 1108 5 0.15692240279705771 1108 6 0.09960642719452295 1108 7 0.15521895025779742 1108 8 0.3121235185049258 1108 9 0.23356137299683866 1108 10-11 0.2664072975606899 1108 12-13 0.15193301328591247 1108 14-15 0.3139843699597833 1108 16-17 0.4010809651473295 1108 18-19 0.32480462818410416 1108 20-21 0.29576944237898317 1108 22-23 0.3705407595067811 1108 24-25 0.3346992424627615 1108 26-27 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-1.1447817773706142 2316 96-97 -0.9636174382768452 2316 98-99 -0.8218825687978217 2316 100-101 -0.8844911035199239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 32.0 5 68.0 6 160.0 7 391.0 8 897.0 9 1559.0 10 2479.0 11 3902.0 12 5388.0 13 6662.0 14 7734.0 15 9290.0 16 10950.0 17 12116.0 18 11235.0 19 10139.0 20 10211.0 21 10871.0 22 12420.0 23 14146.0 24 17407.0 25 21676.0 26 27608.0 27 35141.0 28 43786.0 29 53561.0 30 65411.0 31 78016.0 32 93574.0 33 112535.0 34 136918.0 35 169193.0 36 214383.0 37 270779.0 38 222813.0 39 35676.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.77201035856435 21.3139550824237 14.257486792946134 23.65654776606581 2 15.888536361648994 22.071514578087353 39.18033753937015 22.859611520893505 3 18.490646787173564 26.714823157913 30.89443260452783 23.90009745038561 4 13.096011500669618 18.15324005912316 38.564325362505386 30.186423077701836 5 12.756091396832723 38.12571604383504 35.69488276978045 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27.26321374921022 28.932302171483432 21.89989980437998 28-29 21.908596709365874 27.387184454789924 28.926004106063672 21.77821472978053 30-31 22.189156183678552 27.21517797680378 28.907772935310895 21.687892904206777 32-33 21.98074102011162 27.343858459744368 28.798462706044592 21.87693781409942 34-35 22.17301953897934 27.319924830947716 28.816422899376075 21.690632730696862 36-37 22.220124194865935 27.150414585306258 28.779781827645284 21.84967939218252 38-39 22.10828931087858 27.345527666554954 28.735701316813334 21.810481705753133 40-41 22.177481735519624 27.1949835391958 28.840514542907254 21.787020182377326 42-43 22.07310545342657 27.352240072342582 28.53523910270443 22.039415371526417 44-45 22.06476755185155 27.42127885015516 28.524576510621614 21.98937708737168 46-47 22.17829856391808 27.384283491709127 28.25807832227691 22.17933962209588 48-49 22.12267051018148 27.55641643912311 28.15567391740646 22.165239133288953 50-51 22.212343464419522 27.603682777732875 28.035451124628498 22.148522633219105 52-53 22.378087483198897 27.54209461386824 27.96340237769982 22.116415525233045 54-55 22.12545121002809 27.498787470207343 28.198714327980852 22.17704699178372 56-57 22.181150516106698 27.64847195226942 27.899346107720014 22.271031423903867 58-59 22.197712571888843 27.61919773715545 28.071419585000555 22.11167010595515 60-61 22.23316781855808 27.683785482898976 28.036050454666572 22.046996243876375 62-63 22.264980456341885 27.64001464508109 27.97896433806983 22.116040560507194 64-65 22.06382023564635 27.4788757954953 28.02299790762341 22.434306061234942 66-67 22.047941747887563 27.64043168066468 27.953042396960036 22.35858417448772 68-69 22.081178162998896 27.6005464749759 28.011357880325455 22.306917481699745 70-71 22.122053808469076 27.387942918185765 27.989040226836163 22.500963046508996 72-73 22.06860311619797 27.60062710547074 27.81602822784832 22.514741550482974 74-75 22.08942747506379 27.674507108918196 27.710592988093396 22.525472427924615 76-77 22.03257714380167 27.562078975069817 27.84577329393376 22.559570587194752 78-79 22.025065051263113 27.60113960443511 27.786157600167954 22.58763774413382 80-81 22.04786385782243 27.736435619343446 27.619209274471867 22.59649124836226 82-83 22.113729735731734 27.6848131338367 27.723473702716706 22.477983427714857 84-85 22.10942126545266 27.69053848360352 27.575915824635427 22.624124426308388 86-87 22.017439762380636 27.76245234658376 27.590315946776222 22.629791944259384 88-89 21.993091971621855 27.591139564438542 27.602995117536246 22.812773346403358 90-91 22.023194333512816 27.622650217490254 27.61452433513799 22.739631113858938 92-93 22.06546061583962 27.553395817528877 27.607652776287512 22.773490790344 94-95 21.86287499501192 27.427111894295546 27.699591064371937 23.010422046320592 96-97 21.83524062661929 27.375557498704566 27.7459413631653 23.043260511510844 98-99 21.76809788844474 27.393514277518694 27.567009724998147 23.27137810903842 100-101 21.776714829002888 27.430150732844773 27.52597805166926 23.267156386483084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 269.0 1 202.5 2 222.5 3 309.0 4 243.5 5 198.5 6 298.0 7 519.5 8 797.5 9 932.0 10 839.0 11 720.0 12 781.5 13 914.0 14 1018.0 15 1140.0 16 1274.5 17 1434.5 18 1625.5 19 1848.5 20 2145.5 21 2580.5 22 3156.5 23 3928.5 24 5125.5 25 6862.0 26 9069.0 27 11866.0 28 15127.0 29 19338.0 30 24429.0 31 29630.5 32 35057.0 33 39324.5 34 42459.0 35 45187.5 36 48806.0 37 53114.0 38 55452.5 39 56091.0 40 56549.0 41 57155.5 42 58094.0 43 59971.5 44 61271.5 45 62527.0 46 63327.0 47 63564.5 48 64029.5 49 63093.0 50 61383.0 51 59238.0 52 57495.5 53 55906.0 54 53400.0 55 49161.0 56 43729.0 57 38643.0 58 34237.0 59 29633.5 60 24428.0 61 19847.0 62 16147.0 63 12573.5 64 9413.0 65 7126.5 66 5296.5 67 4102.5 68 3206.0 69 2457.5 70 1908.0 71 1426.5 72 1100.5 73 922.0 74 748.0 75 600.5 76 431.0 77 271.0 78 176.0 79 125.0 80 89.0 81 60.0 82 36.0 83 18.5 84 11.0 85 7.0 86 5.5 87 2.0 88 1.5 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01972064919683174 2 0.0028915907913243024 3 0.0038747316603745647 4 0.032212321415352727 5 0.0019084499222740395 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8915907913243024E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.674772373972906E-5 24-25 3.759068028721593E-4 26-27 0.0019951976460137683 28-29 1.156636316529721E-4 30-31 0.0069687338070915684 32-33 0.02313272633059442 34-35 0.014400122140795026 36-37 8.385613294840477E-4 38-39 8.963931453105338E-4 40-41 0.002573515804278629 42-43 0.009050679176845067 44-45 0.008877183729365608 46-47 0.008212117847361018 48-49 0.010351895032941002 50-51 0.18659435376415723 52-53 0.048752220741727734 54-55 0.018997751499000667 56-57 0.011421783625730993 58-59 0.020501378710489303 60-61 0.05248237286253609 62-63 0.0930803075727293 64-65 0.09776468465467465 66-67 0.10201532311792137 68-69 0.09929722777407654 70-71 0.09030438041305795 72-73 0.07014999259752756 74-75 0.23713936079650605 76-77 0.2855156747353616 78-79 0.007575967873269672 80-81 0.001069888592789992 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.602431712191872E-4 90-91 0.00630366792508698 92-93 0.019749565104744984 94-95 0.0016771226589680954 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1729152.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.97054471724003 #Duplication Level Percentage of deduplicated Percentage of total 1 83.21482548219701 57.39371842057195 2 10.17643041031146 14.037478973525358 3 2.8571632710948984 5.911803214605195 4 1.2216471427006912 3.3703067553730612 5 0.6779305883096528 2.3378620978097877 6 0.43278682190780055 1.7909725712054494 7 0.28662756368238296 1.383820143870455 8 0.20774241199588314 1.1462485852983488 9 0.14511829258990097 0.9007998919525159 >10 0.7409012675515995 8.639255477593883 >50 0.027747615404352697 1.274209191430959 >100 0.010743489380077728 1.3474100607887618 >500 8.39107185485563E-5 0.03478208147511276 >1k 2.517321556456689E-4 0.4313325344991937 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.178353320008883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.1578808572063069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.783181582648605E-5 0.0 0.0 0.0 0.0 7 5.783181582648605E-5 0.0 0.0 0.0 0.0 8 5.783181582648605E-5 0.0 0.0 0.0 0.0 9 5.783181582648605E-5 0.0 0.0 0.0 0.0 10-11 5.783181582648605E-5 0.0 0.0 0.0 0.0 12-13 5.783181582648605E-5 0.0 0.0 0.0 1.445795395662151E-4 14-15 5.783181582648605E-5 0.0 0.0 0.0 1.7349544747945813E-4 16-17 5.783181582648605E-5 0.0 0.0 0.0 2.8915907913243025E-4 18-19 5.783181582648605E-5 0.0 0.0 0.0 2.8915907913243025E-4 20-21 5.783181582648605E-5 0.0 0.0 0.0 3.4699089495891626E-4 22-23 5.783181582648605E-5 0.0 0.0 8.674772373972906E-5 3.4699089495891626E-4 24-25 5.783181582648605E-5 0.0 0.0 2.602431712191872E-4 3.4699089495891626E-4 26-27 5.783181582648605E-5 0.0 0.0 5.783181582648605E-4 4.048227107854023E-4 28-29 5.783181582648605E-5 0.0 0.0 0.001185552224442964 4.048227107854023E-4 30-31 5.783181582648605E-5 0.0 0.0 0.0049157043452513136 4.3373861869864535E-4 32-33 5.783181582648605E-5 0.0 0.0 0.014747113035753942 5.204863424383744E-4 34-35 5.783181582648605E-5 0.0 0.0 0.029783385150640315 5.783181582648605E-4 36-37 5.783181582648605E-5 0.0 0.0 0.05337876600784662 5.783181582648605E-4 38-39 5.783181582648605E-5 0.0 0.0 0.09015980087349175 6.361499740913466E-4 40-41 5.783181582648605E-5 0.0 0.0 0.12974567880672144 6.939817899178325E-4 42-43 5.783181582648605E-5 0.0 0.0 0.16768334998889628 6.939817899178325E-4 44-45 5.783181582648605E-5 2.8915907913243024E-5 0.0 0.21551026167740026 7.228976978310756E-4 46-47 5.783181582648605E-5 8.674772373972906E-5 0.0 0.2736023206751055 7.807295136575616E-4 48-49 5.783181582648605E-5 1.156636316529721E-4 0.0 0.33574260678066475 8.096454215708046E-4 50-51 5.783181582648605E-5 1.156636316529721E-4 0.0 0.4036082426530461 8.096454215708046E-4 52-53 5.783181582648605E-5 1.156636316529721E-4 0.0 0.47179195351247316 8.385613294840477E-4 54-55 5.783181582648605E-5 1.156636316529721E-4 0.0 0.5486793526537863 8.674772373972907E-4 56-57 5.783181582648605E-5 1.156636316529721E-4 0.0 0.6391572285143238 8.674772373972907E-4 58-59 5.783181582648605E-5 1.156636316529721E-4 0.0 0.739726756236583 9.253090532237768E-4 60-61 5.783181582648605E-5 1.156636316529721E-4 0.0 0.8510819176104819 9.831408690502627E-4 62-63 5.783181582648605E-5 1.156636316529721E-4 0.0 0.9600659745354949 9.831408690502627E-4 64-65 5.783181582648605E-5 1.156636316529721E-4 0.0 1.0782163742690059 0.0010988045007032348 66-67 5.783181582648605E-5 1.156636316529721E-4 0.0 1.214728375527426 0.001185552224442964 68-69 5.783181582648605E-5 1.156636316529721E-4 0.0 1.3684742579021392 0.0012722999481826931 70-71 5.783181582648605E-5 1.156636316529721E-4 0.0 1.543097425790214 0.0012722999481826931 72-73 5.783181582648605E-5 1.156636316529721E-4 0.0 1.7287953864090606 0.001330131764009179 74-75 5.783181582648605E-5 1.156636316529721E-4 0.0 1.9177319287141907 0.001387963579835665 76-77 5.783181582648605E-5 1.156636316529721E-4 0.0 2.1354687153009104 0.001387963579835665 78-79 5.783181582648605E-5 1.156636316529721E-4 0.0 2.381687671182175 0.0014457953956621512 80-81 5.783181582648605E-5 1.156636316529721E-4 0.0 2.6410344492560514 0.0014457953956621512 82-83 5.783181582648605E-5 1.156636316529721E-4 0.0 2.9234272059367825 0.0014457953956621512 84-85 5.783181582648605E-5 1.156636316529721E-4 0.0 3.2070344307498706 0.0014457953956621512 86-87 5.783181582648605E-5 1.156636316529721E-4 0.0 3.5209455270560364 0.0015614590273151233 88-89 5.783181582648605E-5 1.156636316529721E-4 0.0 3.869989451476793 0.0016771226589680954 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2140 0.0 51.028378 1 GTATCAA 3615 0.0 38.35061 1 TCAACGC 4495 0.0 30.729855 4 ATCAACG 4535 0.0 30.25034 3 CAACGCA 4625 0.0 29.857458 5 TATCAAC 4830 0.0 28.600965 2 AACGCAG 4875 0.0 28.42365 6 TAACGCA 160 6.287155E-8 26.700521 4 ACGCAGA 5640 0.0 24.315899 7 GTACATG 4645 0.0 23.81595 1 TACATGG 4620 0.0 23.735855 2 CGCAGAG 5765 0.0 23.706354 8 ACATGGG 4665 0.0 23.505533 3 TGGTATC 1080 0.0 22.417196 2 GTGGTAT 1110 0.0 21.814484 1 TATACCG 155 3.937279E-5 21.430779 5 CTAACGC 115 0.0037381554 20.63868 3 TCTAACG 140 4.8726355E-4 20.345018 2 GCAGAGT 6475 0.0 20.22744 9 CATGGGG 3165 0.0 19.79691 4 >>END_MODULE