FastQCFastQC Report
Sat 18 Jun 2016
SRR1930899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495758
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43930.17601866847667122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.050.2456051
GTATCAA35650.045.5454671
ATCAACG43650.037.0647933
TCAACGC45450.036.244824
TATCAAC44850.035.6338422
CAACGCA45700.034.914235
AACGCAG49650.031.3984436
GTACATG59000.027.8600251
ACGCAGA57650.026.977447
TACATGG61100.026.4847412
CGCAGAG61100.025.7068468
ACATGGG63250.025.3415263
CTAACGC2201.1732482E-925.0445563
GTGGTAT7850.022.982251
GCAGAGT69300.021.7234279
CATGGGG46400.021.1505684
GAGTACA44350.020.1134261
AGTACAT44350.019.8816992
GTACTAG5800.019.8728221
CTAGTAC6600.018.9731463
Produced by FastQC (version 0.11.5)