FastQCFastQC Report
Sat 18 Jun 2016
SRR1930899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495758
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.13018089093573976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.11503519171329912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.052.4581871
GTATCAA50700.042.5158041
ATCAACG60800.034.507763
CAACGCA61500.034.0296175
TCAACGC62050.033.73614
AACGCAG65100.032.6567736
TATCAAC65750.031.9826722
ACGCAGA78350.026.6495517
CGCAGAG80000.026.0999058
GTACATG78550.025.1448841
TACATGG81050.023.9539432
GTGGTAT12550.023.8341311
ACATGGG80600.023.086023
GCAGAGT90700.022.6546239
AGAGTAC82600.019.84925710-11
CATGGGG59050.019.3729254
GAGTACA60400.019.3375171
CAGAGTA88500.018.9281310-11
TGGTATC14050.018.2441752
AGTACAT60550.018.1878192
Produced by FastQC (version 0.11.5)