##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930899_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2495758 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193719102573247 31.0 31.0 33.0 30.0 34.0 2 31.31782087846658 31.0 31.0 34.0 30.0 34.0 3 31.397953247069626 31.0 31.0 34.0 28.0 34.0 4 35.26357843989682 37.0 35.0 37.0 33.0 37.0 5 35.0478483891467 35.0 35.0 37.0 32.0 37.0 6 35.08436835622685 36.0 35.0 37.0 32.0 37.0 7 34.958901864683995 36.0 35.0 37.0 32.0 37.0 8 34.93748792951881 36.0 35.0 37.0 32.0 37.0 9 36.43994049102517 38.0 35.0 39.0 32.0 39.0 10-11 36.36024586518404 38.0 35.0 39.0 32.0 39.0 12-13 36.24794290952889 38.0 35.0 39.0 32.0 39.0 14-15 37.25437201844089 39.0 36.0 40.0 32.0 41.0 16-17 37.208673877835906 39.0 36.0 40.0 31.5 41.0 18-19 37.18365081870918 39.0 36.0 40.0 31.0 41.0 20-21 37.13337170510923 39.0 36.0 40.0 31.0 41.0 22-23 36.99475670317395 39.0 36.0 40.0 31.0 41.0 24-25 36.86077015479866 39.0 35.5 40.0 30.5 41.0 26-27 36.571561225086725 38.0 35.0 40.0 30.0 41.0 28-29 36.372336380370214 38.0 35.0 40.0 30.0 41.0 30-31 36.1244728054563 38.0 34.5 40.0 29.5 41.0 32-33 35.89895154097473 38.0 34.0 40.0 29.0 41.0 34-35 36.124114196969416 38.0 35.0 40.0 30.0 41.0 36-37 36.18558309739967 38.0 35.0 40.0 30.0 41.0 38-39 36.14734441400168 38.0 35.0 40.0 30.0 41.0 40-41 36.041537681137356 38.0 35.0 40.0 29.5 41.0 42-43 35.894550873922874 38.0 34.5 40.0 29.0 41.0 44-45 35.70645871915467 38.0 34.0 40.0 28.0 41.0 46-47 35.476334644625 38.0 34.0 40.0 27.0 41.0 48-49 35.26029366629297 38.0 34.0 40.0 27.0 41.0 50-51 34.97491703923217 38.0 33.0 40.0 26.5 41.0 52-53 34.74601884477582 38.0 33.0 40.0 26.0 41.0 54-55 34.54583777754093 37.0 33.0 40.0 26.0 41.0 56-57 34.25997192035446 37.0 33.0 40.0 25.0 41.0 58-59 33.98316222967131 37.0 32.5 40.0 25.0 41.0 60-61 33.65191076218127 36.0 32.0 39.0 24.0 41.0 62-63 33.32122705807214 36.0 31.5 39.0 23.0 40.5 64-65 32.99267457021074 35.5 31.0 39.0 23.0 40.0 66-67 32.63627042365486 35.0 31.0 38.5 22.0 40.0 68-69 32.24756907520681 35.0 31.0 38.0 22.0 40.0 70-71 31.828544073584055 35.0 30.0 37.5 20.5 40.0 72-73 31.394656853749442 34.0 30.0 37.0 20.0 39.0 74-75 30.865398608358664 34.0 29.5 36.0 18.0 39.0 76-77 29.539581561994392 32.5 28.0 35.0 17.5 37.0 78-79 30.306017450409854 34.0 29.0 35.0 18.0 37.5 80-81 30.310145655147654 34.0 29.0 35.0 18.0 37.0 82-83 30.041735616994917 34.0 29.0 35.0 18.0 37.0 84-85 29.723421701943856 34.0 29.0 35.0 15.5 36.0 86-87 29.38283619645815 34.0 29.0 35.0 11.0 36.0 88-89 29.033397869504974 33.5 29.0 35.0 7.0 35.5 90-91 28.67840752188313 33.0 29.0 35.0 3.5 35.0 92-93 28.298889756138216 33.0 27.5 35.0 2.0 35.0 94-95 27.85378370018247 33.0 27.0 35.0 2.0 35.0 96-97 27.356326414660394 33.0 26.5 35.0 2.0 35.0 98-99 26.82355941561642 33.0 25.5 35.0 2.0 35.0 100-101 25.546078586144972 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5190298990755231 1101 2 0.6035673505185599 1101 3 0.5531003404786716 1101 4 0.4220124078432832 1101 5 0.499387323242388 1101 6 0.4483269838956545 1101 7 0.5201015137336427 1101 8 0.5365236779636646 1101 9 0.7525240282709191 1101 10-11 0.676176537006782 1101 12-13 0.718023548438822 1101 14-15 0.6963089919332432 1101 16-17 0.8104417297910587 1101 18-19 0.7635279461805169 1101 20-21 0.7661561671941683 1101 22-23 0.7675190959925544 1101 24-25 0.7750619753999857 1101 26-27 0.8234399267201056 1101 28-29 0.8966752448390167 1101 30-31 0.8184622682538105 1101 32-33 0.6810175767468181 1101 34-35 0.7717820190734699 1101 36-37 0.906174128072351 1101 38-39 0.9712652928099672 1101 40-41 0.9669450335846648 1101 42-43 0.944539550610827 1101 44-45 0.9491368129066586 1101 46-47 0.8372256882849243 1101 48-49 0.9970553141025675 1101 50-51 0.9703990112366867 1101 52-53 1.0150680872084266 1101 54-55 1.0472419237862738 1101 56-57 1.1937480590059764 1101 58-59 0.9625162455670662 1101 60-61 1.0556776656406015 1101 62-63 0.9974234335322976 1101 64-65 1.0710311301788522 1101 66-67 1.0855570607689842 1101 68-69 1.1823350502966719 1101 70-71 0.9740859712553949 1101 72-73 0.943512376870487 1101 74-75 1.1100739400090909 1101 76-77 1.1424998040930525 1101 78-79 1.1540289027668855 1101 80-81 1.07633513339443 1101 82-83 1.3191543520771596 1101 84-85 1.2755823939298558 1101 86-87 1.1917832591726523 1101 88-89 1.202388585964453 1101 90-91 1.2383141025036828 1101 92-93 1.4829649185932574 1101 94-95 1.4863597534446527 1101 96-97 1.3625678574078854 1101 98-99 1.406705076117749 1101 100-101 1.7134156132602705 1102 1 0.47169449180237777 1102 2 0.5618000178476379 1102 3 0.5646202565999303 1102 4 0.3287280579611007 1102 5 0.46958368375570814 1102 6 0.4542112293357974 1102 7 0.502524636740219 1102 8 0.49212741322273956 1102 9 0.6320560304085205 1102 10-11 0.6037383301858128 1102 12-13 0.6701531297156507 1102 14-15 0.7406059622336443 1102 16-17 0.6946417898011958 1102 18-19 0.7316807106095311 1102 20-21 0.7831156711825429 1102 22-23 0.8245826491756958 1102 24-25 0.7853988246776638 1102 26-27 0.8262517339534199 1102 28-29 0.9125526019813393 1102 30-31 0.8977180611632676 1102 32-33 0.881623841778989 1102 34-35 0.9447015262316683 1102 36-37 0.9828339741727348 1102 38-39 1.061107864386969 1102 40-41 0.900992054175731 1102 42-43 0.934935082496466 1102 44-45 0.9550786978890144 1102 46-47 1.0049491702009377 1102 48-49 0.9980550993935822 1102 50-51 1.119537228962443 1102 52-53 0.9340573023150043 1102 54-55 0.9486340101683695 1102 56-57 0.8296328395252957 1102 58-59 0.946689212960429 1102 60-61 1.0300674213803447 1102 62-63 0.78758400469885 1102 64-65 0.8395232973646785 1102 66-67 1.0481753454405904 1102 68-69 1.0149974349752497 1102 70-71 0.9389826772673153 1102 72-73 0.9841080215968674 1102 74-75 1.1226866368284867 1102 76-77 1.244720179501897 1102 78-79 1.1411051149752218 1102 80-81 1.1175839811115722 1102 82-83 1.2558016542959756 1102 84-85 1.27382963708677 1102 86-87 1.0473970186999395 1102 88-89 1.2326368152926186 1102 90-91 1.1417900175060147 1102 92-93 1.2597420757243363 1102 94-95 1.2851461396756427 1102 96-97 1.308839608545945 1102 98-99 1.4506008047635177 1102 100-101 1.7881817531787334 1103 1 0.46262456326496704 1103 2 0.4847155928301987 1103 3 0.4523473947005492 1103 4 0.3099106786755996 1103 5 0.37384420813294383 1103 6 0.3666531463168141 1103 7 0.484092533795895 1103 8 0.5049178825628289 1103 9 0.5439747526960232 1103 10-11 0.5928319991231774 1103 12-13 0.5125459493905922 1103 14-15 0.624099332388603 1103 16-17 0.6594074479416179 1103 18-19 0.6336217791960408 1103 20-21 0.6583170563872756 1103 22-23 0.6333499032091012 1103 24-25 0.7154364518419172 1103 26-27 0.804640524839364 1103 28-29 0.8910049275261542 1103 30-31 0.928944430609782 1103 32-33 0.9055893592074185 1103 34-35 0.7398863313992834 1103 36-37 0.866736963190263 1103 38-39 0.729990785402741 1103 40-41 0.8970223246096225 1103 42-43 0.9022592820275719 1103 44-45 0.8577865219957914 1103 46-47 0.9414734351517282 1103 48-49 0.8298673302697139 1103 50-51 0.9713650471890389 1103 52-53 0.7319103597356857 1103 54-55 0.943107037298553 1103 56-57 0.8685810093474018 1103 58-59 0.9120554329723447 1103 60-61 0.9830285840062842 1103 62-63 0.9145657842876815 1103 64-65 0.7987145181089019 1103 66-67 0.7970174822230476 1103 68-69 0.8188093246492514 1103 70-71 0.6874743902322358 1103 72-73 0.7255331480694949 1103 74-75 0.7991519481252638 1103 76-77 1.0821834690810732 1103 78-79 1.1496888711496815 1103 80-81 1.0859252098298349 1103 82-83 0.989302578091273 1103 84-85 1.0577816230567976 1103 86-87 1.0634885102360663 1103 88-89 1.032612147300199 1103 90-91 1.0805289772571847 1103 92-93 0.9644203693013829 1103 94-95 1.001228802809866 1103 96-97 1.008932286424006 1103 98-99 1.13678827499648 1103 100-101 1.3664514352260788 1104 1 0.4556552451241167 1104 2 0.48720499477994395 1104 3 0.4195289601370149 1104 4 0.24687852295053148 1104 5 0.31027711330293073 1104 6 0.41507308782872343 1104 7 0.5520899741467389 1104 8 0.46222454634538224 1104 9 0.6575369169673877 1104 10-11 0.63759948352034 1104 12-13 0.5982178519924517 1104 14-15 0.6274477383867065 1104 16-17 0.6597747215441458 1104 18-19 0.7862897681007013 1104 20-21 0.6814206999110723 1104 22-23 0.5927741889142979 1104 24-25 0.6468803473861726 1104 26-27 0.8118686973092508 1104 28-29 0.7710844632689913 1104 30-31 0.7319476709941313 1104 32-33 0.7817135795959729 1104 34-35 0.8119298783053353 1104 36-37 0.9268296046760867 1104 38-39 0.8826052273801395 1104 40-41 0.7799975032230861 1104 42-43 0.9200360666247249 1104 44-45 0.8969217709306818 1104 46-47 0.9076507306183643 1104 48-49 0.8052539019727618 1104 50-51 0.8544713097407453 1104 52-53 0.8533608876765797 1104 54-55 0.782171879976616 1104 56-57 0.9034477464203334 1104 58-59 0.9651440303397365 1104 60-61 0.9517510815622146 1104 62-63 0.8275296279179329 1104 64-65 0.964814700698625 1104 66-67 0.9593044343743671 1104 68-69 0.8841776638886572 1104 70-71 0.9629993091696143 1104 72-73 1.0631143167285302 1104 74-75 1.1010599947969766 1104 76-77 1.1117927991825063 1104 78-79 1.2185629436214676 1104 80-81 1.1295276509335146 1104 82-83 1.2406001632839043 1104 84-85 1.4556412569442614 1104 86-87 1.3431334941083684 1104 88-89 1.4248374250550029 1104 90-91 1.4082384252678857 1104 92-93 1.5098183555127882 1104 94-95 1.50975230726009 1104 96-97 1.4386519444022845 1104 98-99 1.700254372899348 1104 100-101 1.9053972817870957 1105 1 0.1419070397569797 1105 2 0.2089238472268633 1105 3 0.2243058458982219 1105 4 0.17436560475627516 1105 5 0.10913171061999094 1105 6 0.14022493326999097 1105 7 0.19758594636866889 1105 8 0.2393943918751944 1105 9 0.3614699299899442 1105 10-11 0.3127717261104337 1105 12-13 0.24408882605907678 1105 14-15 0.392951679727247 1105 16-17 0.3478152196351374 1105 18-19 0.39726106240783565 1105 20-21 0.36017812352453404 1105 22-23 0.42028902032948423 1105 24-25 0.3715499346575015 1105 26-27 0.4033875944506988 1105 28-29 0.5049245981115362 1105 30-31 0.47887782103703813 1105 32-33 0.4547831238593574 1105 34-35 0.3449753676366214 1105 36-37 0.2986957171587221 1105 38-39 0.350587633126338 1105 40-41 0.3164923243501079 1105 42-43 0.17543314166303503 1105 44-45 0.1015862088442887 1105 46-47 0.14766574650389686 1105 48-49 0.3553617117090013 1105 50-51 0.3595658534243853 1105 52-53 0.23720992980116762 1105 54-55 0.2617311170916281 1105 56-57 0.34826779297009836 1105 58-59 0.28858496609979056 1105 60-61 0.2338076781160936 1105 62-63 0.1686391326730572 1105 64-65 0.09892634952612411 1105 66-67 0.08131113960762804 1105 68-69 0.20365261914141541 1105 70-71 0.24806909693385748 1105 72-73 0.26054264209625444 1105 74-75 0.3626235179485313 1105 76-77 0.383948082096758 1105 78-79 0.3936442686877193 1105 80-81 0.3628566711746579 1105 82-83 0.44705686229788455 1105 84-85 0.524395196703626 1105 86-87 0.6002216350900866 1105 88-89 0.7079039967106624 1105 90-91 0.5027168854570796 1105 92-93 0.5912624051321451 1105 94-95 0.5532313071567891 1105 96-97 0.4049402536209499 1105 98-99 0.5578764764515078 1105 100-101 0.7930306011358184 1106 1 0.05017524699632503 1106 2 0.12187914888063389 1106 3 0.14875966625961112 1106 4 0.03666467565022202 1106 5 0.11216294325690512 1106 6 0.07725767186961718 1106 7 0.04259245719035931 1106 8 -0.017294485238679158 1106 9 0.15207621942224847 1106 10-11 0.11479850092610633 1106 12-13 0.08913421946502353 1106 14-15 0.16653812348855013 1106 16-17 0.14755395548456107 1106 18-19 0.13914425590688495 1106 20-21 0.08616759143916397 1106 22-23 0.20972467582014076 1106 24-25 0.12368230920888124 1106 26-27 0.2364118335855636 1106 28-29 0.13060356847925192 1106 30-31 0.13973453576436157 1106 32-33 0.23268365738204722 1106 34-35 0.21003134783170907 1106 36-37 0.13174328584413786 1106 38-39 0.2850781211054425 1106 40-41 0.16622293508019226 1106 42-43 0.30050366662619155 1106 44-45 0.07929991825356097 1106 46-47 0.038008680170747766 1106 48-49 0.13212754416099415 1106 50-51 0.1285582528177116 1106 52-53 0.14485976987307936 1106 54-55 0.2611973530643965 1106 56-57 0.24120084305945255 1106 58-59 0.2632532306172024 1106 60-61 0.2292282482366801 1106 62-63 0.13403510754793757 1106 64-65 0.09211505931081376 1106 66-67 0.01622853154622561 1106 68-69 0.10915211822646853 1106 70-71 0.14087718644210057 1106 72-73 0.3250697655144066 1106 74-75 0.35107472003714335 1106 76-77 0.23223159030339602 1106 78-79 0.21976081561390615 1106 80-81 0.29931032742472397 1106 82-83 0.40676739888827385 1106 84-85 0.3776718991172423 1106 86-87 0.3964616087464634 1106 88-89 0.30605114771667274 1106 90-91 0.38996637435221615 1106 92-93 0.5478958544517809 1106 94-95 0.549006379183421 1106 96-97 0.35734153476187913 1106 98-99 0.4516664182509835 1106 100-101 0.7515002469771517 1107 1 0.09130751521204417 1107 2 0.16858391420703 1107 3 0.22991717005793433 1107 4 0.1044311967325342 1107 5 0.13570361886063864 1107 6 0.1955540644365854 1107 7 0.1251039928383264 1107 8 0.0974378682499335 1107 9 0.18171017361728303 1107 10-11 0.12158651553937005 1107 12-13 0.13446736811255988 1107 14-15 0.14944526368670807 1107 16-17 0.17956713707253869 1107 18-19 0.0761683833747071 1107 20-21 0.24389823405915934 1107 22-23 0.3157149343658219 1107 24-25 0.23496722456363273 1107 26-27 0.30373778706205457 1107 28-29 0.2466312475415151 1107 30-31 0.15523542745047791 1107 32-33 0.20714250455311145 1107 34-35 0.022766714007374844 1107 36-37 0.23981694084818628 1107 38-39 0.2307326122167268 1107 40-41 0.14243390758076657 1107 42-43 0.22174554514045042 1107 44-45 0.17933135451747972 1107 46-47 0.30325364495779894 1107 48-49 0.2681904307567038 1107 50-51 0.2633377174164693 1107 52-53 0.034155694954129956 1107 54-55 0.25815761243251956 1107 56-57 0.23062140399927245 1107 58-59 0.354238760196516 1107 60-61 0.29744696996568365 1107 62-63 0.2133616248211112 1107 64-65 0.27569715936145656 1107 66-67 0.2635387290157354 1107 68-69 0.3530451775755452 1107 70-71 0.3459798710232853 1107 72-73 0.3440601636565823 1107 74-75 0.4489022361286139 1107 76-77 0.2876727693202028 1107 78-79 0.4663474649951347 1107 80-81 0.515942031843764 1107 82-83 0.530860698040307 1107 84-85 0.5591829120899767 1107 86-87 0.42966677121946617 1107 88-89 0.5776017617248037 1107 90-91 0.5271398105468812 1107 92-93 0.5479031589193006 1107 94-95 0.5743865631910765 1107 96-97 0.4637566698350106 1107 98-99 0.4831772147950524 1107 100-101 0.7209802879254568 1108 1 0.2378769470784583 1108 2 0.30869400697048377 1108 3 0.22064479686151017 1108 4 -0.08075553312313133 1108 5 0.08050530775921061 1108 6 0.04979222727990873 1108 7 0.2141449624172722 1108 8 0.2577599585257744 1108 9 0.25460194429386007 1108 10-11 0.22715700396039296 1108 12-13 0.23943169946442566 1108 14-15 0.30985963226198265 1108 16-17 0.34044594708109344 1108 18-19 0.43498981997122144 1108 20-21 0.46982012335130463 1108 22-23 0.46229982869900965 1108 24-25 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-1.1061763672710576 2316 72-73 -0.8749408892811346 2316 74-75 -0.5670887320563729 2316 76-77 -1.0561001661452956 2316 78-79 -0.9718190910229794 2316 80-81 -1.0490040688606328 2316 82-83 -1.1314863290665578 2316 84-85 -1.2232395565198395 2316 86-87 -1.0927590701409642 2316 88-89 -1.2398515374800496 2316 90-91 -1.0706645326118398 2316 92-93 -1.3942962323106336 2316 94-95 -1.1676472168477225 2316 96-97 -1.0232726106651597 2316 98-99 -0.9273298360048159 2316 100-101 -0.8237964024849731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 26.0 4 53.0 5 105.0 6 213.0 7 563.0 8 1343.0 9 2113.0 10 3321.0 11 4874.0 12 6652.0 13 8699.0 14 10309.0 15 12403.0 16 14781.0 17 16673.0 18 15913.0 19 14868.0 20 15240.0 21 16426.0 22 18087.0 23 21462.0 24 26470.0 25 33127.0 26 42042.0 27 53190.0 28 66573.0 29 81224.0 30 98741.0 31 116825.0 32 139068.0 33 166392.0 34 202839.0 35 251416.0 36 320362.0 37 389007.0 38 285473.0 39 38875.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10802618058706 20.478109906527674 14.117671575660326 24.29619233722494 2 16.326360523365445 21.13736700092239 38.51822378865358 24.01804868705859 3 19.39761044422914 25.079076200848345 29.659966325999903 25.863347028922618 4 13.616748888521915 17.132113980815078 36.89397789709789 32.35715923356511 5 13.734568519579598 37.58285217433116 34.78845699219861 13.894122313890636 6 32.33702947160742 36.97922635127284 16.898032581684603 13.785711595435135 7 27.92362079977305 31.752477603998464 21.921877040963107 18.402024555265374 8 25.888968401583806 35.863132563333465 19.85529045684718 18.39260857823555 9 25.994587616267285 15.521897555772634 19.98683365935319 38.4966811686069 10-11 25.274646019365658 26.09151207769343 28.011529964042985 20.622311938897923 12-13 25.52621287801141 23.396799689713507 28.25261904399385 22.824368388281236 14-15 22.72135359277622 25.06274646820725 25.42542185580493 26.790478083211593 16-17 22.741587926393507 28.021486858902183 26.262602383724705 22.974322830979606 18-19 22.65486076775072 27.210791270628004 27.873876393464432 22.26047156815685 20-21 23.353846807262563 26.392482764755236 27.80241513800617 22.451255289976032 22-23 23.110999365923973 26.190264879500514 27.695960044195 23.002775710380515 24-25 23.038133942103403 26.366181607277195 27.747033197535405 22.848651253084 26-27 22.95243359529719 26.56574777302004 27.591972674605785 22.889845957076982 28-29 22.883818416616684 26.665656952980598 27.622000058499285 22.828524571903436 30-31 23.12403717214265 26.45907552096638 27.60744652993284 22.80944077695813 32-33 23.028459817738376 26.577545754272442 27.444267367546516 22.94972706044267 34-35 23.165637204348087 26.580986228040317 27.57740102624974 22.675975541361865 36-37 23.17175686759414 26.414298267089197 27.517136179486428 22.89680868583023 38-39 23.114152550658158 26.498448561313083 27.543265586108856 22.844133301919907 40-41 23.11899531495578 26.443503495501773 27.59956845904418 22.837932730498263 42-43 23.091091489802064 26.448895915676413 27.43037098940325 23.02964160511827 44-45 23.07473917385151 26.582728652648015 27.343007422866144 22.999524750634333 46-47 23.20641416672193 26.512233732318695 27.167843733927565 23.113508367031805 48-49 23.262404896179977 26.584449226837226 27.062450297966755 23.090695579016042 50-51 23.19754589504623 26.65818471860445 26.955253609444224 23.189015776905098 52-53 23.2279370556764 26.655848294783528 26.991523827009743 23.12469082253033 54-55 23.12725990299637 26.62413949606605 27.045076329491728 23.203524271445847 56-57 23.18508237644735 26.71418487419877 26.862554045549437 23.23817870380444 58-59 23.111916207621388 26.72935999503049 27.012802503992646 23.14592129335548 60-61 23.175883337352214 26.777864435976394 27.038961512735447 23.00729071393594 62-63 23.13366692614557 26.718185460438693 26.96220391031094 23.1859437031048 64-65 22.966549682365663 26.58260187229694 27.047678138026505 23.403170307310894 66-67 22.98911774827393 26.68018725879721 26.935738883714887 23.394956109213975 68-69 23.035855773147066 26.640644724891565 26.987176132007612 23.336323369953764 70-71 22.981172791914165 26.456625716274278 27.004560373973526 23.557641117838035 72-73 23.094972364957776 26.58818043360022 26.956179878674053 23.36066732276795 74-75 22.99441926408187 26.73893441882228 26.84139183031671 23.42525448677914 76-77 22.96175274647671 26.649598774615622 26.828407813995202 23.560240664912463 78-79 22.9009788553147 26.631532380604334 26.865727561821593 23.601761202259368 80-81 22.917643749650654 26.735755156844483 26.81264628697781 23.533954806527053 82-83 23.00345225779102 26.609210508390635 26.94163456553079 23.44570266828755 84-85 22.99762236563 26.680190948000565 26.637959289322122 23.684227397047312 86-87 22.78886815147943 26.71008166657184 26.85618958248356 23.644860599465172 88-89 22.836258803707498 26.522862343120963 26.816941984431548 23.823936868739988 90-91 22.8539114232191 26.55384029732627 26.869559119641767 23.72268915981287 92-93 22.87465151364259 26.477909777175125 26.83683093775367 23.810607771428618 94-95 22.649027612160378 26.444617808465082 26.93363657345078 23.97271800592376 96-97 22.67461428551967 26.359506811157175 26.926088186434743 24.039790716888415 98-99 22.563545824555106 26.37693638565919 26.837097186506064 24.222420603279645 100-101 22.556473824785897 26.43052731875446 26.738850481497007 24.274148374962635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 169.0 1 121.0 2 95.0 3 122.0 4 106.5 5 115.0 6 194.5 7 355.0 8 583.0 9 686.0 10 630.5 11 631.5 12 675.0 13 725.5 14 858.0 15 1032.0 16 1160.0 17 1345.5 18 1543.0 19 1714.0 20 2058.5 21 2507.0 22 3013.0 23 3741.5 24 4879.0 25 6492.5 26 8840.0 27 11574.0 28 14596.5 29 18678.0 30 23410.0 31 28686.5 32 35067.0 33 40760.5 34 45605.0 35 50576.0 36 57115.0 37 64355.5 38 69081.5 39 72029.0 40 74212.5 41 75723.5 42 77680.5 43 81950.5 44 86312.0 45 90045.0 46 93418.0 47 95943.0 48 98544.0 49 99533.0 50 98135.0 51 96462.5 52 96291.5 53 95413.5 54 92348.5 55 86396.0 56 78130.0 57 69295.5 58 61359.0 59 54152.0 60 46006.5 61 37908.5 62 30718.5 63 23908.5 64 18379.5 65 14164.5 66 10679.0 67 8344.5 68 6516.0 69 5023.0 70 3937.5 71 3028.5 72 2373.5 73 1978.5 74 1561.0 75 1247.5 76 945.0 77 632.5 78 412.5 79 272.5 80 200.5 81 131.0 82 79.0 83 52.5 84 26.5 85 12.0 86 8.5 87 6.5 88 7.0 89 5.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018992226009092228 2 0.00256435119110106 3 0.00256435119110106 4 0.03533996485236149 5 0.0019232633933257953 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0033993680477033E-5 24-25 6.21053804094788E-4 26-27 0.002303909273254859 28-29 4.0067987360954066E-5 30-31 0.007192203731291254 32-33 0.02438137030914055 34-35 0.014484577430984896 36-37 6.01019810414311E-4 38-39 4.0067987360954064E-4 40-41 0.0020234333617281802 42-43 0.009155535111978004 44-45 0.00905536514357562 46-47 0.008794923225729417 48-49 0.010477778694889488 50-51 0.1837117220499744 52-53 0.04952403237813923 54-55 0.01965334780054797 56-57 0.011759954290440019 58-59 0.021756917136998056 60-61 0.05142726177778455 62-63 0.0921163029428334 64-65 0.0963034076220531 66-67 0.10113160009904806 68-69 0.0992684386867637 70-71 0.08865042203611087 72-73 0.06941778810285293 74-75 0.23812404888615002 76-77 0.2832606366482648 78-79 0.007612917598581273 80-81 8.614617282605124E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.2037393048524734E-4 90-91 0.007332441687054594 92-93 0.02013416364887942 94-95 0.001843127418603887 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2495758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.96331569547825 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6869806018748 46.583689432587136 2 12.711222534980479 15.244140994810234 3 4.1204578403313405 7.412289428690902 4 1.784796015087875 4.280891476189833 5 0.9779605901601537 2.932087980275476 6 0.6087305293307049 2.1900900542239583 7 0.4061618312058459 1.7048367075634785 8 0.2833904894185071 1.3594426705678424 9 0.2180641874685665 1.1768266543550046 >10 1.1172746835290017 11.815498489786522 >50 0.06212827746733031 2.535089553402748 >100 0.02202866224109045 2.2118674560553764 >500 6.028176781219944E-4 0.23820837901664926 >1k 2.0093922603958028E-4 0.31504072247486387 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3249 0.13018089093573976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2871 0.11503519171329912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.202039620828622E-4 2 0.0 0.0 0.0 0.0 1.202039620828622E-4 3 0.0 0.0 0.0 0.0 1.202039620828622E-4 4 0.0 0.0 0.0 0.0 1.202039620828622E-4 5 0.0 0.0 0.0 0.0 1.202039620828622E-4 6 4.0067987360954066E-5 0.0 0.0 0.0 1.6027194944381626E-4 7 4.0067987360954066E-5 0.0 0.0 0.0 2.0033993680477032E-4 8 4.0067987360954066E-5 0.0 0.0 0.0 2.0033993680477032E-4 9 4.0067987360954066E-5 0.0 0.0 1.6027194944381626E-4 2.0033993680477032E-4 10-11 4.0067987360954066E-5 0.0 0.0 1.803059431242933E-4 2.0033993680477032E-4 12-13 4.0067987360954066E-5 0.0 0.0 2.804759115266785E-4 4.0067987360954064E-4 14-15 4.0067987360954066E-5 2.0033993680477033E-5 0.0 2.804759115266785E-4 5.008498420119258E-4 16-17 1.202039620828622E-4 4.0067987360954066E-5 0.0 4.4074786097049473E-4 6.41087797775265E-4 18-19 1.202039620828622E-4 4.0067987360954066E-5 0.0 4.607818546509718E-4 6.811557851362192E-4 20-21 1.202039620828622E-4 4.0067987360954066E-5 0.0 4.808158483314488E-4 7.212237724971732E-4 22-23 1.4023795576333922E-4 8.013597472190813E-5 0.0 4.808158483314488E-4 7.612917598581273E-4 24-25 1.6027194944381626E-4 8.013597472190813E-5 0.0 5.60951823053357E-4 8.013597472190813E-4 26-27 1.6027194944381626E-4 8.013597472190813E-5 0.0 7.212237724971732E-4 9.415977029824205E-4 28-29 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.0014624815386748233 9.616316966628976E-4 30-31 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.005569450243172615 0.0010217336777043288 32-33 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.016868622678961664 0.001121903646106714 34-35 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.03533996485236149 0.001202039620828622 36-37 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.06362796392919506 0.001242107608189576 38-39 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.10249391166932051 0.001242107608189576 40-41 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.14456529839832227 0.001242107608189576 42-43 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.19002243005932468 0.001242107608189576 44-45 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.24473526680070745 0.0013022095892310072 46-47 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.30832316274254157 0.0013623115702724384 48-49 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.37365401613457716 0.0013623115702724384 50-51 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.4465376851441526 0.0013623115702724384 52-53 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.5191208442485209 0.0013623115702724384 54-55 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.5986557991600148 0.0013823455639529153 56-57 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.6966019942638669 0.0014424475449943464 58-59 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.8037437924670581 0.0014825155323553005 60-61 1.6027194944381626E-4 8.013597472190813E-5 0.0 0.9197205818833396 0.0014825155323553005 62-63 1.6027194944381626E-4 8.013597472190813E-5 0.0 1.0402050198777286 0.0015626515070772085 64-65 1.6027194944381626E-4 8.013597472190813E-5 0.0 1.16752104971716 0.0016027194944381626 66-67 2.0033993680477032E-4 8.013597472190813E-5 0.0 1.3109444104756953 0.0016227534881186397 68-69 2.0033993680477032E-4 8.013597472190813E-5 0.0 1.477366795979418 0.0016427874817991168 70-71 2.404079241657244E-4 8.013597472190813E-5 0.0 1.659055084667664 0.0016427874817991168 72-73 2.804759115266785E-4 8.013597472190813E-5 0.0 1.8505399962656637 0.0016427874817991168 74-75 2.804759115266785E-4 8.013597472190813E-5 0.0 2.0452704148399006 0.0016427874817991168 76-77 2.804759115266785E-4 8.013597472190813E-5 0.0 2.26490308755897 0.0016427874817991168 78-79 2.804759115266785E-4 8.013597472190813E-5 0.0 2.512483181462305 0.0016628214754795937 80-81 2.804759115266785E-4 8.013597472190813E-5 0.0 2.7877502546320594 0.001702889462840548 82-83 2.804759115266785E-4 8.013597472190813E-5 0.0 3.077381701270716 0.001803059431242933 84-85 2.804759115266785E-4 8.013597472190813E-5 0.0 3.3768698728001674 0.0018230934249234098 86-87 2.804759115266785E-4 8.013597472190813E-5 0.0 3.6968327858710657 0.001883195405964841 88-89 2.804759115266785E-4 8.013597472190813E-5 0.0 4.0599288873360315 0.0019232633933257953 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2670 0.0 52.458187 1 GTATCAA 5070 0.0 42.515804 1 ATCAACG 6080 0.0 34.50776 3 CAACGCA 6150 0.0 34.029617 5 TCAACGC 6205 0.0 33.7361 4 AACGCAG 6510 0.0 32.656773 6 TATCAAC 6575 0.0 31.982672 2 ACGCAGA 7835 0.0 26.649551 7 CGCAGAG 8000 0.0 26.099905 8 GTACATG 7855 0.0 25.144884 1 TACATGG 8105 0.0 23.953943 2 GTGGTAT 1255 0.0 23.834131 1 ACATGGG 8060 0.0 23.08602 3 GCAGAGT 9070 0.0 22.654623 9 AGAGTAC 8260 0.0 19.849257 10-11 CATGGGG 5905 0.0 19.372925 4 GAGTACA 6040 0.0 19.337517 1 CAGAGTA 8850 0.0 18.92813 10-11 TGGTATC 1405 0.0 18.244175 2 AGTACAT 6055 0.0 18.187819 2 >>END_MODULE