FastQCFastQC Report
Thu 26 May 2016
SRR1781506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332637
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20751562.38482189293434No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG60311.8130875398707902No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT4490.13498197735068557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA252.831508E-670.02093553
CGGCCGA207.914507E-570.0103918
ACGTATC308.337302E-658.36836655
GGTCGAC308.35967E-658.34199550
TCGACGT308.35967E-658.34199552
TGGTCGA308.367137E-658.33320649
CGTATCA8800.057.71378756
GTATCAT8800.056.51970757
GCCGTAT8750.056.4338754
CCGTATC8700.056.35566355
ATCATTA8900.056.2951759
TCGCCGT8650.056.25112552
GTGGTCG9300.056.06670848
CGCCGTA8700.055.93626453
GTCGCCG8900.055.85098651
TGGTCGC8900.055.84257549
CGGTGGT9550.055.33186346
TCGGTGG9550.055.3068845
GGTCGCC9000.055.23042350
TCTCGGT9200.055.12142643
Produced by FastQC (version 0.11.5)