SRR1781506_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781506_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332637
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT	161474	48.54360759626861	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG	15203	4.570447665172545	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	7726	2.322652020069926	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC	7458	2.242083712876198	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC	2610	0.7846391111030945	No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT	1589	0.47769791093594516	TruSeq Adapter, Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC	1255	0.37728815495570245	No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT	1208	0.3631586383956084	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT	1189	0.35744670616918744	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT	1023	0.30754245619098297	RNA PCR Primer, Index 38 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG	919	0.276277142951626	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT	682	0.20502830412732195	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG	595	0.17887366709055216	No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC	568	0.17075671076879603	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT	549	0.165044778542375	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT	478	0.14370018969627552	No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC	470	0.14129516560094035	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG	456	0.13708637343410385	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT	400	0.12025120476675775	No Hit
CTTATACACATCTCCGAGCCAACGAGACTCAGCTATATCTCGTATGCCGT	397	0.11934932073100707	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC	384	0.11544115657608744	RNA PCR Primer, Index 29 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTAAGCAGT	381	0.11453927254033676	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCAGT	375	0.1127355044688354	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGGG	15	0.002222885	70.00497	69
GTGACGA	15	0.002222885	70.00497	42
GTTTGCG	20	7.917566E-5	70.00496	55
TTGCGTG	20	7.917566E-5	70.00496	57
TTTGCGT	20	7.917566E-5	70.00496	56
ACGACTA	20	7.917566E-5	70.00496	45
ACTACGC	15	0.002224211	69.99444	27
AGGCGAG	15	0.0022255378	69.98392	25
GACAACG	20	7.92937E-5	69.98391	18
CGACATG	20	7.92937E-5	69.98391	15
AGGGGGG	18655	0.0	67.15298	70
AACTCGT	90	0.0	66.12574	37
ACTCGTA	85	0.0	65.896935	38
TAACTCG	80	0.0	65.63952	36
ATATCGT	45	3.8380676E-10	62.23599	37
CGTAATC	40	1.0633812E-8	61.25434	48
TACGAGA	40	1.0659278E-8	61.235924	13
AAGGGGG	20535	0.0	61.005054	69
ACGTATG	35	2.968045E-7	60.004253	40
GCCGTAT	165	0.0	59.398148	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content