FastQCFastQC Report
Thu 26 May 2016
SRR1781505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236322
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20129985.17996631714357No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG52432.2185831196418446No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT4020.17010688805951202No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3250.13752422542124726No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2440.10324895693164411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAAA150.002207100270.1244461
GCAGTAA150.002207100270.1244460
AGTAAAA150.002207100270.1244462
TCATGAA150.002207100270.1244460
GTAAAAA150.002207100270.1244463
GGATCAT207.871025E-570.07979657
CGGATCA252.8148734E-670.07979656
GCGGATC150.00221454770.06492655
TGGCGGC207.9124926E-570.00551619
TGGGGAC150.002222012570.0055119
GCGGCTG150.002225752569.9758458
CGAAGAT150.00222762469.9610125
CACGTCT150.002229496869.94627
CGGGTCT150.002229496869.94627
CGCGGCT150.002229496869.94627
CGCGTCG150.002229496869.94627
GCGGGTC150.002229496869.94626
GATGTGT150.00223137169.9313833
CTCGGGG150.00223137169.9313844
TGCGGGT207.9624835E-569.916585
Produced by FastQC (version 0.11.5)