FastQCFastQC Report
Thu 26 May 2016
SRR1781505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236322
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT16176668.45151953690304No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG148916.301148433070133No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC74093.1351291881416032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67852.8710826753328087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC24481.035874781019118No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT14400.6093381064818341TruSeq Adapter, Index 9 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT13460.5695618689753811No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC12410.5251309653777473No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG9560.40453279846988427No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5810.24585099990690668No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC5510.23315645602186846No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC5230.22130821506249945No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT4370.18491718925872325No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT4300.18195512901888103No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC4280.1811088260932118RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT4200.17772361439053494No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG4190.17730046292770035No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT3990.1688374336710082No Hit
CTTATACACATCTCCGAGCCAACGAGACGTATTCGGATCTCGTATGCCGT3530.14937246638061627No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT3520.14894931491778168No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3410.144294648826601No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC3330.14090943712392415No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT3260.1379473768840819No Hit
CTTATACACATCTCAGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3070.12990749909022437No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2640.11171198618833626No Hit
CTTATACACATCTCCGAGACCACGAGACGTATTCGGATCTCGTATGCCGT2530.10705732009715559No Hit
GCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG2530.10705732009715559No Hit
CTTATACACATCTCCGAGCACACGAGACGTATTCGGATCTCGTATGCCGT2400.1015563510803057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT2650.070.041291
CTTAAAC900.070.041281
TATACGC301.0195981E-770.011643
ACGCATC301.0195981E-770.011646
TTATACG353.6925485E-970.011642
TACGCAT301.0195981E-770.011645
ATACGCA301.0195981E-770.011644
TTAAACA950.070.011642
ACTCGTA700.069.99682638
AATCCGA150.00222311169.99682611
TAGGATC550.069.99682633
TATACGG650.069.99682630
AAATCGT301.0210897E-769.99682637
AAATCGG207.917388E-569.99682630
AAATCCG150.00222311169.99682610
TGCCGAC900.069.99682645
AGCTAGA252.834773E-669.99682655
CACAACA207.917388E-569.9968267
TACGGAT700.069.99682632
TACGGAA150.00222311169.99682632
Produced by FastQC (version 0.11.5)