FastQCFastQC Report
Thu 26 May 2016
SRR1781504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738749
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21203728.702170832041734No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG56510.7649418137960254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC200.00695238452.4974623
CACGCGA200.006967310352.46899833
GTACGCG200.006980390852.444124
CGTATCA8700.049.913156
CCGTATC8600.049.67907355
CGCCGTA8600.049.67907353
TCGCCGT8750.048.8241252
GCCGTAT8900.048.00449854
GTGGTCG9350.047.90562448
GTCGCCG9000.047.8537351
CGGTGGT9800.046.76759346
CTCGGTG9350.046.76381344
ACGCGTC305.895167E-446.6391076
GGTCGCC9350.046.43060750
TGGTCGC9500.046.06289349
TCGGTGG9800.046.0442245
TCTCGGT9500.046.01608343
GATCTCG9750.045.53812841
ATCTCGG9850.044.7208942
GTATCAT9900.043.86600557
Produced by FastQC (version 0.11.5)