FastQCFastQC Report
Thu 26 May 2016
SRR1781504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738749
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT16828722.77999699491979No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG152442.0634884108134157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC81981.1097138540965876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74861.0133347050215973No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC27270.3691375555161496No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT17970.24324906023561452TruSeq Adapter, Index 2 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT13650.1847718237182047No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC13520.1830120920637456No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG10160.13752979699464907No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC9630.13035550640339275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG196600.066.45759670
AAGGGGG217500.060.1037469
CCGTCTA850.057.6473447
GCCGTCT222950.057.0293846
CCGTCTT222500.056.99128747
TATGCCG224650.056.97942743
ATGCCGT224300.056.95524644
TGCCGTC223750.056.9506145
AAAAGGG230250.056.94272667
CGTCTTC223550.056.8958248
AAAGGGG230250.056.86672268
AATCTCG225750.056.8528836
CGTATGC227150.056.83385541
CTCGTAT226500.056.8191939
GTATGCC225150.056.77900342
TCGTATG228700.056.77009640
TCTCGTA226150.056.70588338
GCTTGAA228500.056.6282856
ATCTCGT226350.056.6248437
TGCTTGA227200.056.5517755