FastQCFastQC Report
Thu 26 May 2016
SRR1781503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123787
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2144417.323305355166536No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG7070.5711423655149571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC150.002221725269.9878810
GGCGCGT150.002225292169.959585
TCGACTC200.006920015452.53338658
GTGCGCC200.006942203752.49090211
GATTTAG200.00695331852.469681
GTGCCAA200.00695331852.469681
GGCGTGC352.0787116E-549.9913338
CCGTATC1100.047.73830455
AAGGCGT305.863965E-446.658586
GCCGTAT1150.045.66272754
TCGCCGT1100.044.55574852
CGTATCA1100.044.55574856
GTCGCCG1150.042.61854651
GGTCGCC1150.042.61854650
CGCCGTA1150.042.61854653
CTCGGTG1150.042.6013144
TGGTCGC1150.042.6013149
ATCTCGG1150.042.5840942
GTGGTCG1250.041.9927248
TCTCGGT1200.040.80975743
Produced by FastQC (version 0.11.5)