FastQCFastQC Report
Thu 26 May 2016
SRR1781503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123787
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT98237.935405171787021Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT13551.0946222139643096Illumina PCR Primer Index 6 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG13331.0768497499737453No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11110.897509431523504No Hit
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC8930.7214004701624565Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC8730.7052436847164888No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT6310.50974658082028Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC5120.4136137074167724Illumina PCR Primer Index 6 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC3720.30051620929499867Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2970.2399282638726199No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT2820.22781067478814415Illumina PCR Primer Index 6 (95% over 23bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2590.20923037152528134No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC2170.1753011220887492No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT1310.10582694467108825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT207.900737E-570.037
AACTCGT352.954166E-760.037
CGTATGA553.45608E-1157.27272441
TATGACG452.6773705E-854.44444343
ATTATAC1500.053.666671
TCTTTAT1650.053.0303041
ACACCGA200.006937460552.511
CCGGACA407.429717E-752.528
ATCGTAT200.006937460552.539
GCCGTAT200.006937460552.546
TAACTGG200.006937460552.564
AGGGGGG18950.052.45382770
ACCGGAC352.076591E-550.027
AGTATGC506.141454E-848.99999641
TATATCG305.8589765E-446.66666836
CCGTATT305.8589765E-446.66666847
ATATCCG305.8589765E-446.66666810
TCTCGAA305.8589765E-446.66666838
TATCGTA305.8589765E-446.66666838
TTTACAC1050.046.6666642
Produced by FastQC (version 0.11.5)