FastQCFastQC Report
Thu 26 May 2016
SRR1781502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864830
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22370825.867280274736075No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG55620.6431321762658557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC150.002229215669.9643544
CGCCGTA8400.052.92953553
CCGTATC8400.052.53406555
GTCGTAT200.006981429652.442913
CGTATCA8350.052.43528756
GTCGCCG8750.051.6065751
GCCGTAT8650.051.4146754
TCGCCGT8650.050.99210752
GTGGTCG9350.048.66366648
GATCTCG8950.048.45010441
ATCTCGG9000.048.18372342
GGTCGCC9400.048.03803650
TCTCGGT9050.047.9230643
TGGTCGC9500.047.89806749
CGGTGGT9750.047.0044146
CTCGGTG9600.045.91410444
TCGGTGG9900.045.58547245
CCGGCGA503.479603E-642.00048421
GTAGATC10250.041.96646538
ATCATTA10400.041.7699559
Produced by FastQC (version 0.11.5)