FastQCFastQC Report
Thu 26 May 2016
SRR1781502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864830
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT17528620.268260814264075No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG163471.8901980736098425No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC93011.0754714799440352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74790.8647942370176798No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC33470.3870124764404565No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT24480.2830614109131274RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC19270.2228183573650313No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT14380.1662754529791982No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG13110.15159048599146652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG198850.066.1076270
AAGGGGG220650.059.59211769
CGTCTTC227050.056.2167648
GCCGTCT227400.056.21043846
ATGCCGT229350.056.20885544
TGCCGTC228600.056.16359345
CCGTCTT226900.056.1459547
TATGCCG230200.056.1446543
TCGTATG232250.056.10126540
CTCGTAT232000.056.0776739
CGTATGC231200.056.0564641
TCTCGTA232450.055.9841738
GCTTGAA231400.055.916256
GTATGCC231350.055.880742
ATCTCGT233150.055.83110437
CATCTCG233950.055.70004736
TGCTTGA230050.055.69664455
AAAGGGG236550.055.61614668
GTCTTCT229200.055.58574749
CTGCTTG229400.055.56458354