FastQCFastQC Report
Thu 26 May 2016
SRR1781501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347695
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21563562.01843569795366No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG52651.514258186053869No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT4370.1256848674844332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC150.00221825670.0424164
GCGGGTA252.8411869E-669.9818434
GCCACGG150.002225880769.9818432
TGCGCGT207.9593636E-569.931455
TGCGGGT352.990073E-759.9412465
CGGATCA308.337822E-658.36867556
CGCCGGA308.344954E-658.36025253
CCGGCGA308.359235E-658.34342621
GCGCGTC308.387857E-658.30986
CCGGATC252.3815641E-456.0339255
ATTGGAC252.3866614E-456.00968620
TCGCCGT5900.054.59358252
TGGTCGC6350.054.57637449
GTCGCCG6300.054.4538651
GTGGTCG6700.054.32992648
CCGTATC5950.054.1504355
CGCCGTA5950.054.1426253
GGTCGCC6350.054.02509750
GCCGTAT6000.053.6991854
CGTATCA6200.053.09666456
Produced by FastQC (version 0.11.5)