FastQCFastQC Report
Thu 26 May 2016
SRR1781501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347695
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT16367547.07430362817987No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG150604.331382389738132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC80792.323588202303743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69592.0014668028013056No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC30700.8829577647075741No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT21080.6062784912063734TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC20440.5878715540919485No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT17780.5113677217101196No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13960.40150131580839526No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT12950.3724528681746933No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT12240.3520326723133781No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG11670.33563899394584334No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC9680.2784049238556781No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC8470.24360430837371835No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG6670.19183479773939804No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG6150.17687916133392773No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC5770.16595004242223788No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT5660.1627863501056961No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT5070.14581745495333553No Hit
CTTATACACATCTCCGAGCCAACGAGACCATCGACCATCTCGTATGCCGT3540.10181337091416329No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT401.3278623E-1070.0695637
CAACACG150.002214852370.0695636
ATCGTAA207.881972E-570.0695639
TCGACCG150.002219917570.0292131
GACGTAT207.921412E-569.9989946
ACGTCAT207.921412E-569.9989947
ACGTATT207.921412E-569.9989947
TAAGACG150.00222372269.9989943
GCAAACG252.841918E-669.9788618
TACGAGA207.9327096E-569.9788613
TCAACGA207.9327096E-569.9788611
AGGGGGG195750.066.9236870
TCGAACA750.065.360631
ACGTCTT1650.063.6354547
ACTCGTA1000.063.062638
TCGAAAA501.4551915E-1163.02629531
ATCGAAA501.4551915E-1163.0081630
ATGACGT2000.062.99909644
AATCGTA453.8016879E-1062.28405438
CGTATGA2700.062.2481941
Produced by FastQC (version 0.11.5)