FastQCFastQC Report
Thu 26 May 2016
SRR1781500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358891
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19018352.991855465865676No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG50361.4032115600558388No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT3880.10811081916236406No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG150.002221594870.01633513
CTTCGAA150.00222282570.0065619
TACGTTT150.002227750669.9675131
CGACGAG352.971101E-759.99724622
CGCGTCT308.384508E-658.314397
CCGTATC6050.056.152355
CCCTTCG252.3893503E-455.99743324
CGAAGAT252.3909983E-455.9896225
CGTATCA6150.055.81652556
GCCGTAT6100.055.6920454
GTCGCCG6500.055.47447251
CGCCGTA6150.055.23925853
TCGCCGT6250.054.90046352
TGGTCGC6700.054.3182949
GTGGTCG6900.054.265348
GGTCGCC6600.054.08842550
CGGTGGT7050.053.09590546
TCTCGGT6700.052.72934743
GGTGGTC7100.052.72199247
TCGGTGG7100.052.72199245
Produced by FastQC (version 0.11.5)