FastQCFastQC Report
Thu 26 May 2016
SRR1781500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358891
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT15341342.74640489730865No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG136083.7916804823748715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC74022.06246464804077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65921.8367693812327417No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC20430.5692536173935818No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT13050.3636201520796008RNA PCR Primer, Index 36 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT12090.3368710834208715No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT11550.3218247323003363No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC9590.2672120504554308No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG7020.19560256456695765No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG6300.17554076307291072No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4700.13095898197502862No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4260.11869899217311106No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT4220.117584447645664No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCAGT3920.10922536368981112No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC3820.10643900237119348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTA455.456968E-1270.02830538
CGTATCA150.002222547570.0087941
TCTACGG150.002222547570.0087942
TCCGATC150.00222500669.9892713
TAGACGG150.00222500669.9892724
ACTCCGA301.02336344E-769.9892711
GATACGG207.927216E-569.98926524
CGATACG207.927216E-569.98926523
AGGGGGG174400.068.0629670
CATCACG501.4551915E-1163.0254736
CCGTCAT1300.061.91358647
AAGGGGG192650.061.65159269
AATCTCG2950.060.5329436
TATGACG1700.059.7050543
AAAGGGG203800.058.36445668
ATGACGT1500.058.33251644
TGCCGTC199750.058.2419945
TATGCCG200150.058.2305243
ATGCCGT200150.058.2305244
GCCGTCT198950.058.22989746
Produced by FastQC (version 0.11.5)