FastQCFastQC Report
Thu 26 May 2016
SRR1781499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572411
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19686134.391547332249026No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG57200.9992819844482372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG207.954243E-569.948352
CCGTATC7600.059.8922955
CGTATCA7700.059.5744156
CGCCGTA7700.059.1041353
TCGCCGT7850.058.85636552
GCCGTAT7750.058.72794754
GTGGTCG8650.057.8640948
CTCGGTG8400.057.46761744
TCTCGGT8250.057.2304743
GTCGCCG8350.057.0087751
ATCTCGG8250.056.80653842
GATCTCG8350.056.12622541
CGGTGGT8850.055.7605546
TCGGTGG8850.055.74105545
TGGTCGC8550.055.6752349
GGTCGCC8850.053.78793350
GGTGGTC9250.053.7323547
GTATCAT8550.053.65180657
GTAGATC8900.053.44368438
TTGCGCC200.0069585452.48419627