FastQCFastQC Report
Thu 26 May 2016
SRR1781499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572411
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT15731527.48287506704099No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG142062.481783194243297No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC79241.3843200078265443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74111.2946990885919383No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC26680.466098659879003No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT16600.29000141506714583RNA PCR Primer, Index 36 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT13140.2295553369868853No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC12570.21959745707192907No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG9280.16212127300139237No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC5770.1008016966829778RNA PCR Primer, Index 36 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG5770.1008016966829778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG176500.067.31605570
AAGGGGG195400.060.8407569
ATATCCG1100.060.44835710
ACTCGTA650.059.2402438
CCGTCTA900.058.32736647
CGTATGA2000.057.75418541
AAAGGGG207700.057.2714668
CGTCTTC202600.057.19806348
ATGCCGT202900.057.1874844
GCCGTCT202300.057.17907346
CCGTCTT201450.057.17210847
TGCCGTC202600.057.16850345
TATGCCG203500.057.1048743
CTCGTAT204550.057.0049539
TCGTATG206150.057.0040140
CGTATGC204800.056.99875341
AAAAGGG209200.056.97791767
AATCTCG204500.056.9504236
GCTTGAA205100.056.94951656
GTATGCC204300.056.91552442