FastQCFastQC Report
Thu 26 May 2016
SRR1781498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257610
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14213155.172935833236295No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG29531.1463064322037189No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAG150.002218629270.03422560
GTTAAGT150.002218629270.03422559
ACTACTT150.002223768569.993416
TGCCGGC401.0635631E-861.24421319
GTTATGC252.4001472E-455.9403461
TCGCCGT3950.055.82836552
GTCGCCG4200.055.82806451
CGCCGTA3900.055.65740653
CGTATCA3850.055.48166356
CCGTATC3850.055.48166355
GCCGTAT3900.054.77035554
GGTCGCC4300.054.52973650
ATCTCGG4300.053.7054242
GATCTCG4300.052.89170541
TGGTCGC4450.052.69165449
GCGACGA200.0069485352.4950421
GCGAATG200.0069485352.4950430
GAGCCGT200.0069485352.4950419
GACGATT200.0069485352.4950423
CCCGAGT200.0069485352.4950450
Produced by FastQC (version 0.11.5)