FastQCFastQC Report
Thu 26 May 2016
SRR1781498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257610
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT10826942.02825977252436Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG105754.105042506113893No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC49401.9176274212957571No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38481.4937308334303794No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC19670.7635573153216102No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT14380.5582081440937853Illumina PCR Primer Index 1 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT10770.41807383253755676TruSeq Adapter, Index 1 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT10580.41069834245565Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC9330.36217538139047395Illumina PCR Primer Index 1 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG6790.26357672450603625No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG4680.1816699662280191No Hit
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC4380.17002445557237686Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC4130.16031986335934165Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT3690.1432397810643997No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3680.14285159737587827Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT3570.13858157680214278No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG3050.11839602499902954No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2780.10791506540895153Illumina PCR Primer Index 1 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT2760.10713869803190869Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAGT2740.10636233065486587No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT2640.10248049376965179No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT2610.10131594270408757TruSeq Adapter, Index 1 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC150.002219564770.0268135
ACGAACA150.002219564770.0268134
CGACTAC150.002222987469.9996248
CGTCAAA150.002222987469.9996248
CCGAATT207.917173E-569.9996147
ACCGAGA207.9247926E-569.9860213
CAAGCGC150.002224769.9860213
TCGCAGT150.00222641469.9724422
AGGGGGG127150.067.30202570
TAAGCCG453.8198777E-1062.24605643
TATGACG1300.061.94679643
AAGGGGG142450.060.22079569
ATATCGT1200.058.35567537
CACGTAT308.342751E-658.35567539
CGAGCAA308.37162E-658.32168615
GAGACGG308.381261E-658.31036824
CACGAAC1150.057.83699833
CCGAGAC850.057.63554814
ATGACGT1400.057.52202244
ACGTCTT1400.057.49968347
Produced by FastQC (version 0.11.5)