FastQCFastQC Report
Thu 26 May 2016
SRR1781497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458214
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23552751.4010920661525No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG65721.434264339369814No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT5010.10933755843339575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG150.002224859369.993557
ACCGCAC150.00222871769.9629928
CCGTCAG200.006924920752.5468266
CGGAAAC200.00692791652.54107764
CCCGCGT200.00694290452.51237512
CGCGTGT200.006951908652.4951677
ACTGCCG200.006954911652.48943748
ACGTACA200.006963927852.4722442
GATAGGT200.006963927852.47224435
TGTGCGC407.507351E-752.466523
AACGCTA200.00696693552.4665240
CGTATCA9350.052.0557256
CGCCGTA9450.051.85840253
TCGCCGT9450.051.48798852
GTGGTCG10000.051.4396548
CCGTATC9400.051.40070355
TCTCGGT10100.051.2544243
GCCGTAT9500.051.22259554
CGGTGGT10200.051.11158446
GTCGCCG9600.051.04254551
Produced by FastQC (version 0.11.5)