FastQCFastQC Report
Thu 26 May 2016
SRR1781497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458214
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT18287939.91126416914367No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG160823.5097138018480445No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC95092.0752312238386432No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85671.8696504253471087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC34100.7441937609937715No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT23690.5170073371830629TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC18930.41312574473935765No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT13900.3033517090267866No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG13080.2854561405806021No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT11370.2481373332111197No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT10960.2391895489880274No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7850.17131733207627878No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7800.17022613887834068No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG7020.15320352499050663No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGACGT6180.13487147926514687No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG5670.12374130864617842No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT5260.11479352442308616RNA PCR Primer, Index 25 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC5080.11086522891050907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG207.913934E-570.0171436
TCGTAAT150.002222851670.009540
AAAACGG301.0234908E-769.9942267
TATCCCG150.00222477769.9942243
AACACGA150.00222477769.9942219
AGGGGGG212500.066.3709970
AAGGGGG233200.060.4345469
ACTCGTA900.058.3476138
TATCTCG244750.056.70028736
AAAGGGG249000.056.58568668
ATATCTC245450.056.5813835
TGATATC247200.056.56319833
GATATCT247800.056.454534
CGTCTTC240300.056.4497748
GCCGTCT240700.056.443246
CCGTCTT240400.056.4408447
TGCCGTC241400.056.41000445
ATGCCGT243050.056.40143244
ATGATAT249300.056.3816332
TATGCCG243150.056.3494543
Produced by FastQC (version 0.11.5)