FastQCFastQC Report
Thu 26 May 2016
SRR1781496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363123
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20962757.7289238081862No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG50611.3937426161383333No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT4050.1115324559446799No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAG207.925951E-569.9917225
ACGCGTC150.002225932469.982086
CGTATGC150.002229586569.953151
ACGAACA352.9726834E-759.9929124
TTGTGCG308.4156E-658.278222
CTGCCGG800.056.87611818
GTATCAT6400.056.34477257
CGGCCGA252.388615E-456.00110218
CACGTCT252.3918727E-455.985667
TTGTACG252.4000325E-455.947092
CGTATCA6550.055.58894756
CGCCGTA6400.055.23546253
CCGTATC6350.055.126855
ATCATTA6600.054.11436559
TATCATT6600.054.10689558
TCATTAA6650.053.7074960
CGGTGGT6850.053.64329546
GCCGTAT6550.053.4361754
TGGTCGC6850.053.13240449
GTCGCCG6800.053.01574751
Produced by FastQC (version 0.11.5)