FastQCFastQC Report
Thu 26 May 2016
SRR1781496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363123
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT16461345.33257326030023No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG147024.0487658451819355No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC89302.4592218063851643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66711.8371185521159497No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC29630.8159769554668803No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT20300.5590392236239511Illumina PCR Primer Index 6 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC17370.4783503110516271No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT14390.3962844545787515No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG11360.31284165420532434No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC11090.3054061571423457No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8190.22554341091035268No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8020.2208618016484772No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG6270.17266876512917112No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT5530.15228999540100738Illumina PCR Primer Index 6 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5420.14926071881979386No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT5140.14154983297670487No Hit
CTTATACACATCTCCGAGCCAACGAGACGATCCAAAATCTCGTATGCCGT4440.12227261836898241No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC4270.11759100910710696No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT4250.11704023154688631No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCAGT4070.11208323350490054No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3710.102169237420929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGA150.002217606470.0482537
ACTCGAA150.002217606470.0482538
TATCGAA150.002217606470.0482538
AATAACG150.002217606470.0482536
ACTCGTA950.070.0482438
AATCGTA252.825489E-670.0482438
CGATAAA252.8347567E-670.0096428
GAGCGTA150.0022248969.99036462
ACGACTA150.0022248969.99036447
CCGACAT207.92673E-569.9903647
GCCGTAA207.92673E-569.9903646
CTAGACG301.0242729E-769.9807223
ATAACCG207.93214E-569.9807210
AGGGGGG189400.066.5814570
GACGATA1550.065.46583626
CGATACA1350.064.82374628
ACGATAC1300.064.6064827
AACTCGT1050.063.3769837
CTCGTAA900.062.26510639
TCGTAAG900.062.24794440
Produced by FastQC (version 0.11.5)