FastQCFastQC Report
Thu 26 May 2016
SRR1781495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403248
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21703153.820725707257075No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG54111.341854144347895No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT4220.10465024005078762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCG207.917505E-570.0086916
ACACCGT150.002226043569.982636
GACGCGG252.3877011E-456.00695414
GGCGCGT252.3935662E-455.9791565
TCGCCGT7650.055.83085652
CGTATCA7750.055.5759856
CGCCGTA7750.055.56218753
ATCTCGG7950.054.9973142
GATCTCG7950.054.9973141
CCGTATC7850.054.8611955
GCCGTAT7850.054.8611954
TCTCGGT8050.054.7622243
CTCGGTG8200.054.6138144
GTCGCCG7900.054.5004451
CGGTGGT8250.054.2962946
TCGGTGG8800.053.67319545
CCGGCGG850.053.5294121
GTGGTCG8200.053.34703448
GGTCGCC8100.053.14814850
TGGTCGC8150.052.8155349
Produced by FastQC (version 0.11.5)