FastQCFastQC Report
Thu 26 May 2016
SRR1781495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403248
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT16760241.563008372019205No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG153233.7998948537872472No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC93142.309744871642265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71351.7693826131809705No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC29680.7360234892671508No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT21970.5448260127762568Illumina PCR Primer Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC18370.45555092647700673No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT13280.32932587390390033No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG11520.2856802761576003No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC8500.21078839820656273No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT7790.19318136729754393No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC7030.17433440463436892No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT6910.17135856842439393No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG6600.16367099154862516No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT5420.13440860215053765No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5300.13143276594056263No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT5200.12895290243225013No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT5070.12572907987144386No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4860.12052136650398762No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT4840.12002539380232512No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTAAGCCGT4730.11729754394318136No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCAGT4340.10762607626076261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAT353.6998244E-970.0153832
TTAGACG150.002224104269.998023
GGTGCTA150.002225198669.98933421
CGCTTCA150.002225198669.9893348
AGGGGGG194550.067.4254970
CCGTCTA700.065.00622647
CGGACAT1350.062.23589732
ACTCCGA900.062.21274611
ACGAATG401.0630174E-861.2634640
CACGTAT2350.061.07724439
ACGGACA1500.060.6724731
AAGGGGG216700.060.60682769
TGCCGAC1050.060.00573745
ACCGGTC950.058.9530130
CTACGAG950.058.9383912
ATGCCGA1200.058.33891744
AACTCCG900.058.32444810
ATACGAG308.377832E-658.32444412
ATCACGT2950.058.14837337
TCACGTA2950.058.14837338
Produced by FastQC (version 0.11.5)