FastQCFastQC Report
Thu 26 May 2016
SRR1781494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251495
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14289956.819817491401416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCG150.00222350269.9950217
AAGTACG150.002225258669.98109425
ACGATTG200.00694769752.4962713
ACGCGGC200.00694769752.4962715
CGGGCGC305.8726413E-446.6633512
GTTATTC305.8957364E-446.6262173
TATTAAC404.5972243E-543.729482
CCGGCGG503.4716923E-641.99701321
CGTATCA602.5783265E-740.8548356
TTAGTCT350.001250654140.02105756
GTTAACT350.001251880140.0130969
TGCCGGC350.001254334839.99715419
GTATCAT708.628358E-735.01842557
CTAATGA400.002416689634.99751334
TGATAAT601.2133627E-534.9975114
CGCGGCC601.2133627E-534.9975116
TAGGCCA400.002426147734.9696664
CCGTATC552.9776333E-431.83493255
CGCCGTA552.981116E-431.82859453
TCGCCGT552.9846022E-431.82225452
Produced by FastQC (version 0.11.5)