FastQCFastQC Report
Thu 26 May 2016
SRR1781494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251495
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT10391641.319310523072026No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG89363.5531521501421497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC40661.616731942980974No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT19000.7554822163462495No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC15260.6067715063917771No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT13140.5224755959363009TruSeq Adapter, Index 11 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT11440.4548798186842681No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC9770.38847690808962404No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG7140.28390226445853795No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6810.270780731227261No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT6210.24692339807948468RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT5230.20795642060478342No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC4370.17376090975963737No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC3900.15507266546054593No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT3540.14075826557188018No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG3480.13837253225710253No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT3170.12604624346408477RNA PCR Primer, Index 11 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG3160.12564862124495516No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2800.1113342213562894No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT2790.11093659913715978No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT2690.10696037694586373No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2650.10536988806934532No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC2570.10218891031630849RNA PCR Primer, Index 29 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG150.002219020470.0306536
TCGAGTG150.002219020470.0306538
TAAATCG207.899441E-570.0306536
ACGTATG1050.070.0167140
CGCACTG150.002222526170.00278527
ACGACAC150.00222603669.97494522
AGGGGGG127050.067.3446570
GCACACG850.065.8718718
CGAGCAC850.065.8718715
TATCACG1300.064.6436836
CCGTCTA600.064.15645647
CACGTAT1150.063.94102539
CCGTATT1350.062.21232247
GCCGTCA2400.061.25243846
ACTCCGA1200.061.24025711
CTAATAC4250.060.9557231
CGTCTTA1150.060.85988248
CCGTCAT2300.060.85988247
ACTCGTA750.060.69322638
AACTCGT750.060.69322637
Produced by FastQC (version 0.11.5)