FastQCFastQC Report
Thu 26 May 2016
SRR1781493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156182
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9608561.521174014931304No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1590.10180430523363768No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATATC150.00221498470.05012564
TCGCCGT150.002217809470.02762652
CGTATCA150.002217809470.02762656
CCGTATC150.002217809470.02762655
CGCCGTA150.002217809470.02762653
GTCGCCG150.002220637470.00513551
TGGTCGC150.002220637470.00513549
ACAAGGT150.002237662469.8705144
TGGCGCT308.326748E-658.35635813
CGCTGGC252.379954E-456.022116
CTGGCGC252.379954E-456.022112
GTTGAAT200.006929985752.52071815
TATCAGG200.006929985752.52071823
CACAGCA200.006929985752.5207189
CCCTTGT200.006929985752.52071816
GGCGCTG407.422004E-752.52071814
TGGTCGG200.006929985752.52071810
GTGGTCG200.006938797452.50385348
AATGCTC200.00694761752.48699643
CTATAGC200.006991841452.402884
Produced by FastQC (version 0.11.5)