FastQCFastQC Report
Thu 26 May 2016
SRR1781493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156182
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT6981744.70233445595523No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG69774.467224135943963No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC26091.6704869959406332No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT14190.9085554033115211No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT12320.7888232958983751TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC11460.733759332061313No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT9250.5922577505730494No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC7390.47316592180917133No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT5920.3790449603667516No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG4630.2964490146111588No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC3170.20296833181800722No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC2680.1715946780038673No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG2610.16711272745899014No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2560.16391133421264933No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT2530.16199049826484485No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG2430.15558771177216324No Hit
TCTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG2320.14854464663021347No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT2120.13573907364485024No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2090.13381823769704576No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT1970.12613489390582783No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC1700.10884737037558745No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC1640.10500569847997848RNA PCR Primer, Index 42 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT1600.10244458388290584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT353.6543497E-970.0772934
GAACTCG455.456968E-1270.0772936
AACTCGT455.456968E-1270.0772937
AGGCCGA150.00221440470.05482529
CCGACTA150.00221440470.05482532
CGTAAGC301.012977E-770.05482541
AGCCGTC150.00222286769.9875145
AACCGAC150.00222286769.9875160
CAAACCG150.00222286769.9875158
AGGAGTT150.00222286769.9875156
CGACCAG150.00222286769.9875163
CAACAGT150.00222286769.9875146
CCGTCAT600.069.9875147
CCGACCA150.00222286769.9875162
AAGATTG150.00222286769.9875154
GCCGTCA600.069.9875146
ATCTCGG150.002225693369.965110
TAACCGA150.002225693369.965111
TCTCGGA150.002225693369.965111
CCGAGAA150.002225693369.965114
Produced by FastQC (version 0.11.5)