FastQCFastQC Report
Thu 26 May 2016
SRR1781492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205581
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15115473.52527714137007No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2430.11820158477680331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA150.002217392270.0389956
TGGCGCT252.8315135E-670.00487513
CGGGTCT150.00222168270.004877
AACTTGG150.00222168270.004877
TAAGTGG150.002225978569.970795
CTAACAC150.00223028169.936743
ACTTATA150.002232434569.919722
GGTGCCG252.3855771E-456.003917
GTGCCGG252.3855771E-456.003918
GTAGATA252.3942052E-455.96301338
GTCGGTG252.3942052E-455.96301344
GCTGGCG200.00694203652.50365417
GTGGCGC200.00694203652.50365412
TACTGTA200.00694203652.5036547
CATAGAT200.00694203652.50365412
CACAGAT200.00694203652.5036547
CCGGCGG407.46295E-752.4908721
TAAATAG200.006948726752.4908725
CATACCC200.00696212252.4653243
TGTCGGT200.00696212252.4653243
Produced by FastQC (version 0.11.5)