FastQCFastQC Report
Thu 26 May 2016
SRR1781492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205581
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT11240954.67869112417977No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG98754.80345946366639No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC46542.263827882926924No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT23691.1523438450051318No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT14130.6873203262947452No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT13140.6391641250893808TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC13040.6342998623413643No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT9080.44167505751990704No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC8180.39789669278775763No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC6390.31082638959826053No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG6160.2996385852778224No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC3960.19262480482145722No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3710.18046414795141574No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG3650.17754559030260578No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC3380.164412080882961No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG3380.164412080882961No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3220.1566292604861344No Hit
TCTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG3080.1498192926389112No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT2800.13619935694446472No Hit
CAAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2550.12403870007442323No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT2430.11820158477680331No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT2240.10895948555557178No Hit
CTTATACACATCACCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2200.10701378045636513No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT2170.10555450163196015No Hit
TCATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG2100.10214951770834853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGA252.8234044E-670.0387137
TATCGAA252.8234044E-670.0387138
ATCGAAT252.8234044E-670.0387139
ATCGTAA150.002217430470.03870439
AAACGTA150.002217430470.03870438
ATATAGT207.891514E-570.03870437
AACTCGA353.6779966E-970.03870437
CACGTAA150.002217430470.03870439
AATAACG150.002217430470.03870436
TCGTATC150.002219572770.0216640
GCGAATG207.9105695E-570.0046229
TATGCCC207.9105695E-570.0046243
GCCGAAT150.002221716370.0046246
ATTATAC252.8315826E-670.004621
CACGTCT150.002221716370.0046246
AACCGAG207.9201105E-569.98759512
GATACGG150.002223861669.98759524
TTAGAGA150.002223861669.9875953
CGTCTAA150.002223861669.98759548
CGCGAAT207.9201105E-569.98759528
Produced by FastQC (version 0.11.5)