FastQCFastQC Report
Thu 26 May 2016
SRR1781491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228452
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13240857.95878346436013No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCCG150.002221980870.00503517
CGACGAC150.00222391269.9897122
GATTGCG150.00222391269.9897143
GACGACC150.00222391269.9897123
TGCCGGC252.3887017E-455.99176819
GCCGGCG452.6939233E-854.43644320
TCTTGTC200.00694296452.5037778
TCGAAGA200.00694296452.50377750
CTGTAGG200.00694898152.492281
TTAAGGC200.00694898152.492283
CGCAGGT200.00694898152.4922834
GCTACGT200.00694898152.4922839
GGTTCAT200.00694898152.492281
TATCGAA200.00694898152.4922848
ATCGAAG200.00694898152.4922849
CCGGCGG352.083986E-549.99264521
AACGAAT350.001254563839.99411831
TCCAACG350.001254563839.99411828
GGTATAG556.682405E-638.1762051
ATCCCGT400.002417132334.99485420
Produced by FastQC (version 0.11.5)