FastQCFastQC Report
Thu 26 May 2016
SRR1781491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228452
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT9414941.21172062402606Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG85163.7276977220597765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC40131.7566053262829828No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT20670.9047852502932783Illumina PCR Primer Index 6 (95% over 24bp)
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT18780.8220545234885227Illumina PCR Primer Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC14740.6452121233344422No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT12170.5327158440285049Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC11860.519146253917672Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG7560.33092290721902196No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT7050.30859874284313554Illumina PCR Primer Index 6 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC5500.24075079228897098Illumina PCR Primer Index 6 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT4350.19041199026491343Illumina PCR Primer Index 6 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC4340.18997426155166072Illumina PCR Primer Index 6 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG4160.1820951447131126No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT3830.16765009717577434TruSeq Adapter, Index 6 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT3520.15408050706494145TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT3130.1370090872480871No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3080.13482044368182375Illumina PCR Primer Index 6 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC2850.12475268327701225No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2640.11556038029870608No Hit
TCTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2640.11556038029870608No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT2610.11424719415894806No Hit
CTAAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2510.10986990702642131Illumina PCR Primer Index 6 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT500.070.0000153
TGACGTA252.8337072E-670.0000145
GCAAGAG150.002222617470.03
AACCCAG150.002222617470.07
CCGTCCA150.002222617470.028
GCCGACA207.9150486E-570.046
ACTCGAA207.9150486E-570.038
CGAGCAA353.6961865E-970.015
AACTCGA207.9150486E-570.037
ATACGAG150.002222617470.012
ACGAATG207.9150486E-570.040
CTCGAAT1100.066.81818439
AGGGGGG118100.066.2362470
TGCCGAC850.065.88235545
CCGGCAA550.063.63636828
ATCTACG501.4551915E-1163.00000410
GAATGAC453.8380676E-1062.22221842
TCTCGAA1200.061.2538
AAGGGGG129450.060.45577669
CGGACAA650.059.23076629
Produced by FastQC (version 0.11.5)