FastQCFastQC Report
Thu 26 May 2016
SRR1781490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166255
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14806689.05957715557426No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2190.13172536164325885No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1850.11127484887672552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAAAA207.893037E-570.0280163
AGGAAGA150.002218023970.02862
GGGAGCA150.002218023970.02856
TGCCGGC401.3460522E-1070.0069319
CTGGCGC252.8286377E-670.0069312
GCCGGCG455.456968E-1270.0069320
GCGCTGC401.3460522E-1070.0069315
CTGCCGG353.6852725E-970.0069318
TGGCGCT207.9048215E-570.0069313
CCGGCGG455.456968E-1270.0069321
CGCTGCG150.002220674970.0069216
TCTGGCG150.002220674970.0069211
CGGACGC150.002220674970.0069212
CGGTGCC150.002220674970.0069216
GTCGGGC150.002220674970.0069210
TCGGGCG150.002220674970.0069211
GTGTGCG150.002225984169.96482
GATACGC150.00222864269.9437563
GGCGCTG550.063.64266214
CGTATCA252.3803554E-456.02240456
Produced by FastQC (version 0.11.5)