FastQCFastQC Report
Thu 26 May 2016
SRR1781490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166255
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT10603563.77853297645184No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG97365.8560644792637815No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC39042.3482000541337102No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT23121.3906348681242668No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT19341.1632732850139846RNA PCR Primer, Index 37 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC17431.0483895221196355No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC15480.9310998165468707No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT15260.9178671318155844No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG10130.6093049833087727No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT8930.537126702956302No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC6990.4204384830531413No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC5480.3296141469429491No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5010.3013443204715648No Hit
TATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCT4970.2989383777931491RNA PCR Primer, Index 37 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4350.26164626627770593No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGGAACATCTCGTATGCCGT3920.2357823824847373No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG3640.2189407837358275No Hit
TCTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG3280.19728729963008632No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC3230.1942798712820667No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3150.18946798592523534No Hit
CAAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT2600.15638627409701963No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCAGT2440.1467625033833569No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAAATCTCGTATGCCGTC2130.12811644762563532No Hit
CTTATACACATCACCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT2110.12691347628642746No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGACGT2080.1251090192776157No Hit
TCATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG2060.12390604793840786No Hit
CATAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT1950.11728970557276473No Hit
CTTATACACATCTCCGAGCCAACGAGACATGGGAACATCTCGTATGCCGT1820.10947039186791375No Hit
CTTATACACATCTCAGAGCCCACGAGACATGGGAACATCTCGTATGCCGT1730.10405702084147846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720.10345553517187452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC150.002216969870.0364151
CTCTATA301.01523256E-770.0364151
ATTAAAC150.002216969870.0364151
GTTATAC207.888361E-570.0364151
TCTTAAT252.8215763E-670.0364151
CTCGAAA150.00221961870.0153439
ACACGAA207.9001315E-570.0153438
ACTCGAA353.6816346E-970.0153438
CCTATGC150.00221961870.0153441
AGCTTAT252.8266259E-670.015342
TATACGC207.9001315E-570.015343
TATACAA301.0173608E-770.015343
ACGCATC207.9001315E-570.015346
TCCTAAG150.00221961870.0153440
AAACAAC401.3460522E-1070.015346
GGACCAT150.00221961870.0153432
CCTAAGC150.00221961870.0153441
CAACTCG1450.070.0153436
TCGAAAG150.00221961870.0153440
CTAAGCC207.9001315E-570.0153442
Produced by FastQC (version 0.11.5)