FastQCFastQC Report
Thu 26 May 2016
SRR1781489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186726
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16822690.0924349046196No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130.114070884611677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGG150.00221694770.0399817
GTGGCGC150.00221694770.0399812
CTGCGGG150.00221930870.02119418
GGTGGCC150.002221670770.0024150
GTGGCCG150.002221670770.0024151
GGGCGCT453.8016879E-1062.25776313
CTGCCGG352.956167E-760.0181618
GCGCTGC553.45608E-1157.3054415
CGCTGCG252.3791031E-456.03198616
CATTAAA452.6751877E-854.47554461
TCATTAA452.6751877E-854.47554460
TCGGGCG200.006927288652.52998411
TGCCGGC407.434719E-752.51589219
GAAATAA200.006942006352.5018151
CCGGCGG407.448489E-752.5018121
AGTGTAG200.00695674752.47366735
GGTGTGC200.00695674752.4736671
GTGTGCG200.00697151152.4455532
GTGCGGG352.0901893E-549.961534
GGCGCTG850.049.43998714
Produced by FastQC (version 0.11.5)