FastQCFastQC Report
Thu 26 May 2016
SRR1781489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186726
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT12090864.7515611109326No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG105195.633387958827372No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC50132.684682368818483No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT21931.1744481218469844No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT20011.071623662478712TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC17060.9136381650118356No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC15890.8509795100842945No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13840.7411929779462956No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT9140.4894872701177126No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG8760.4691365958677421No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT7920.42415089489412294No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC7780.4166532780651864No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT7570.4054068528217817No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT7530.40326467658494264No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC5940.3181131711705922No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5460.29240705632852415No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT4790.2565256043614708No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG3560.19065368507867142No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3340.1788717157760569No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC3330.17833617171684715No Hit
CTAAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3200.17137409894712038No Hit
TCTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG3160.1692319227102814No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACATCTCGTATGCCGTC2550.13656373509848657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.12317513361824277No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT2270.12156850144061351No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCAGT2190.11728414896693552No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGAACATCTCGTATGCCGT2060.11032207619720874No Hit
CAAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2060.11032207619720874No Hit
CATAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT1880.10068228313143321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA401.3278623E-1070.0521
TCTTAAT353.6689016E-970.0521
CAATACA150.002215442770.0519941
CTTATAA150.002215442770.0519941
GTTATAC301.01435944E-770.0519941
TTAAACA3800.070.014472
CGACAAA207.903164E-570.01446532
ACCATAA301.01812475E-770.01446534
TAACGTA301.01812475E-770.01446538
CGCCAAT150.00222015870.01446532
TTATAAA150.00222015870.0144652
ATTACAC150.00222015870.0144654
CCATAAC252.828201E-670.01446535
TATTACA150.00222015870.0144653
CATAACG252.828201E-670.01446536
AATCTGC252.8327067E-669.9957251
TACAGCA207.9136604E-569.9957252
AAATCGA207.9136604E-569.9957228
ACGTCTA252.8327067E-669.9957247
TGCCGTA500.069.9957245
Produced by FastQC (version 0.11.5)