FastQCFastQC Report
Thu 26 May 2016
SRR1781488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295649
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17330258.617482217088515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA207.894306E-570.0439156
ACGACTG150.002223709669.9964459
TTATACG207.9741985E-569.901692
TATACGC150.002237191269.889873
GCCGTAT252.3807249E-456.0351354
TCTAGAC252.4067721E-455.9118923
AGTCCGA200.006929733352.53293256
CCAGATA200.006929733352.53293256
CCGTATC200.006929733352.53293255
CAGTCCG200.006929733352.53293255
CGCATCA200.006939032652.51512513
GTTGCGG200.00694368652.50622621
TGCTCCG200.00695299952.48843830
AGCTCGC200.00695299952.4884388
GACGACT200.00695299952.4884388
TGCGCGT200.006962321752.4706575
ATACGCG200.006971653652.4528924
ATCTTGG200.006985668652.4262661
GATGCGG200.006985668652.4262661
GTCGCCG352.080121E-550.0144151
Produced by FastQC (version 0.11.5)