FastQCFastQC Report
Thu 26 May 2016
SRR1781488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295649
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT12916343.68795429715642No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG111263.7632462819086143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC56621.9151087945502945No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT24850.8405237291517983No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC16280.5506529702451217No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT16190.5476088199182139RNA PCR Primer, Index 36 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT15260.5161525998734986No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC10340.34973904866919897No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT9460.3199740232505437No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT8520.28817956428061653No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG8160.2760029629729849No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4890.16539883442866374No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4570.15457518882188No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG4210.14239858751424833No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG4170.14104563181340035No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3620.12244249092674084No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT3500.11838362382419693No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3360.11364827887122907No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC3320.1122953231703811No Hit
TCTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG3190.10789821714262521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG252.8243048E-670.0477536
TTATACG252.835688E-670.000342
AACCGAG207.918699E-570.00033612
GTGTACG150.002223221670.0003361
TATACGA150.002223221670.00033611
ACCGAGA150.002224714369.988513
ACGGTAC207.925339E-569.98849527
AGGGGGG153400.067.7870370
CGTATGA2000.063.032341
CCGACTT950.062.63187847
ACGTCTT1850.062.43273547
AATATCG453.8198777E-1062.26466436
CGGTAAC453.8380676E-1062.21199428
CTTAAAC4100.061.463711
AAGGGGG169850.061.2424569
AGAGACG1150.060.8492723
GCCGTCA1500.060.6669646
GGTAACC352.9682633E-760.0002929
AACTCCG1400.059.9901410
ACTCGTA1350.059.67030338
Produced by FastQC (version 0.11.5)