FastQCFastQC Report
Thu 26 May 2016
SRR1781487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235956
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16285169.01752869178999No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAC150.002210935870.0937361
ATAAGGG150.002214666570.0639455
TATTTGA150.002220271470.0193211
GGACGCT301.0187978E-770.0193213
TGGGACG150.002220271470.0193211
CATCGGA150.002225886869.974759
ATCGGAC150.002225886869.9747510
CACATCG150.002229636369.945077
ACATGGG150.002229636369.945078
ACATCGG150.002229636369.945078
TTGTGCG150.002233390569.9154052
GGTATAC150.002233390569.9154051
TCGGACG252.3838696E-456.01545711
ATATATG200.00691435852.55912857
CGACGAG200.006949295352.492222
GGGTCGC200.00695513152.48106449
TAGCAGG200.00695513152.48106410
GACACCG200.006960970352.4699331
CAAGCCA200.006960970352.4699329
ATTAGCC200.0069726652.4476743
Produced by FastQC (version 0.11.5)