SRR1781487_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781487_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235956
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	120839	51.21251419756226	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	10310	4.3694587126413404	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	4869	2.0635203173473022	No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	2437	1.0328196782450965	No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT	1894	0.8026920273271287	RNA PCR Primer, Index 36 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	1752	0.7425113156690231	No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	1508	0.63910220549594	No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	1155	0.489498042007832	No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	966	0.4093983624065504	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	815	0.34540338029124074	No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	544	0.23055145874654598	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	509	0.21571818474630863	RNA PCR Primer, Index 36 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	406	0.17206597840275306	No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT	405	0.17164217057417486	RNA PCR Primer, Index 36 (95% over 22bp)
CTAAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	376	0.15935174354540677	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC	375	0.15892793571682856	No Hit
TCTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	361	0.15299462611673365	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	356	0.1508755869738426	No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	324	0.1373137364593399	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	313	0.1326518503449796	No Hit
CAAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	272	0.11527572937327299	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT	249	0.10552814931597417	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGT	35	3.6943675E-9	70.006355	37
TCGGCTT	15	0.0022219066	70.006355	53
CGGCTTG	15	0.0022219066	70.006355	54
TTCGGCT	15	0.0022219066	70.006355	52
CCTATGC	15	0.0022219066	70.006355	41
CGATCTC	20	7.912022E-5	70.006355	35
TCGAAAG	45	5.456968E-12	70.006355	40
ACGAAAG	30	1.02012564E-7	70.006355	40
ATCTACG	55	0.0	69.99152	10
ACACCGA	35	3.6998244E-9	69.99152	11
AAGCGGG	45	5.456968E-12	69.99152	69
ATATCCG	30	1.0216172E-7	69.99152	10
TACGCAT	20	7.920334E-5	69.99152	5
TCTACGA	55	0.0	69.99152	11
CTACGAG	65	0.0	69.99152	12
CCGACTA	20	7.920334E-5	69.99152	47
CCGACAA	20	7.920334E-5	69.99152	47
ATACGCA	20	7.920334E-5	69.99152	4
ATACGAG	15	0.0022237755	69.99152	12
CGACTAC	20	7.920334E-5	69.99152	48

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content