FastQCFastQC Report
Thu 26 May 2016
SRR1781486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247603
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14970160.46009135592057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACC150.002221156870.01333663
CGTCTGG352.9624607E-760.0114369
CGCGTCT352.966608E-759.999317
CGACGGG200.00694039152.51000622
TGCGCCA200.00694039152.51000612
GGGCAAT200.00694039152.51000611
TCTGGCG407.4504715E-752.51000611
GCGCGTC407.460876E-752.4993936
CGTGAGA200.00695149452.4887928
TCCCGGC200.00695149452.4887928
GCGTCTG451.6781887E-646.6755568
GTGCGCG604.9312803E-946.6472744
TGCGCGT551.3113095E-744.5269475
GTCGCCG350.001252685440.00762651
TATAAGA459.214453E-538.864882
TCGCCGT400.002413520835.0066752
GTCTGGC601.2111168E-535.00666810
GGGGTCG501.7047035E-435.00666848
GTATAAG501.7047035E-435.0066681
GCGCTGC601.2111168E-535.00666815
Produced by FastQC (version 0.11.5)