FastQCFastQC Report
Thu 26 May 2016
SRR1781486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247603
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT10837943.77127902327516Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG99354.012471577484925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC41701.6841476072583936No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT19860.8020904431691054Illumina PCR Primer Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT16890.6821403617888313Illumina PCR Primer Index 1 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC15460.6243866189020327No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT13050.5270533878830224Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC10540.42568143358521504Illumina PCR Primer Index 1 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT8120.3279443302383251Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT7380.2980577779752265TruSeq Adapter, Index 1 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG7120.287557097450354No Hit
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC5430.2193026740386829Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC4710.19022386643134373Illumina PCR Primer Index 1 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG3910.15791408020096687No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT3520.14216305941365817No Hit
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT3410.13772046380698133Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3280.1324701235445451Illumina PCR Primer Index 1 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3220.13004688957726684Illumina PCR Primer Index 1 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG2880.11631523042935667No Hit
CTAAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2710.10944940085540159Illumina PCR Primer Index 1 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG2580.10419906059296535No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG150.002221235870.0127262
TATACGC150.002221235870.0127263
AATCCGA150.00222301669.9985911
TCCGAAC150.00222301669.9985913
GCAAACG207.917149E-569.9985918
GCCGAAT150.00222301669.9985946
CGTAAGA207.917149E-569.9985941
ACGTAAG150.00222301669.9985940
CGACTTA301.0211079E-769.9985948
ATCGAAT207.917149E-569.9985939
CCGTCTA750.069.9985847
AGGGGGG133150.067.05459670
CGTCTAC800.065.6236748
ATCCGAG750.065.3320112
AACTCGT1500.065.3320137
ATATCCG600.064.1653710
TATCCGA600.064.1653711
ACTCGTA1650.063.63507538
ACACGAA2150.063.4870932
GTATGAC1900.062.63031842
Produced by FastQC (version 0.11.5)