FastQCFastQC Report
Thu 26 May 2016
SRR1781485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294941
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19404365.79044622483818No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG150.002223749669.99618
CGGTCTA150.002223749669.996119
TACGCGT150.002228244469.960485
GGTCTAG308.392753E-658.3003961
CGGGTGT252.390772E-455.9873737
CCGGCGA200.00694846552.4970721
TTCACCG200.00694846552.4970730
TTAAGCC200.00694846552.4970720
CGGTCCG200.00694846552.4970710
TGTACCA200.00694846552.4970717
TATAAGT200.00694846552.4970733
TCAATCT200.006953130552.48816346
CGCGTGT200.006953130552.4881637
GTACGCG200.006967141852.4614564
GTCTAGG352.0906364E-549.971772
TGCGGGT305.8886217E-446.6403165
TGTGCGG305.8935484E-446.6324043
CTGCCGG659.986252E-943.07452418
TGCCGGC503.4733803E-641.99765819
TACAGTC350.00125564139.99098227
Produced by FastQC (version 0.11.5)