FastQCFastQC Report
Thu 26 May 2016
SRR1781485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294941
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT14214648.194723690500815No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG119614.055387348656172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC62382.1149992710406487No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT27130.9198449859463418No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT21500.7289593511922723TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC19420.6584367721001827No Hit
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC16410.5563824629332647No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT16360.5546872086281662No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG10420.35329099718248735No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT10020.3397289627417009No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT8190.2776826551751028No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7800.264459671595336No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC6680.22648597516113392No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT6090.20648197436097387No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG5860.19868380455752166No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT5220.17698454945226333RNA PCR Primer, Index 25 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG4270.14477471765539549No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3840.13019553063155004No Hit
CTAAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3770.1278221746044124No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAAGATATCTCGTATGCCGT3690.12510976771625512No Hit
TCTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG3620.12273641168911748No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGAAATCTCGTATGCCGT3600.12205830996707816No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC3570.12104115738401917No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGACGT3300.11188678413648831No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA150.002219202770.03223438
TAGACCG150.002219202770.03223439
ATCACGA150.002219202770.03223437
ACGTAAG150.002219202770.03223440
CACGTAA150.002219202770.03223439
TCGAAAG252.8280083E-670.03223440
GCCGTCG150.002220696870.02035546
CCGTCGT150.002223686869.9966147
CCGACTA207.920761E-569.9966147
CGTCGTC150.002223686869.9966148
ACGCATC150.00222518369.984746
GACAACG207.927418E-569.9847418
TGTACCG150.00222518369.984745
ATACCGC150.00222518369.9847426
AAAGCGG500.069.9847468
ACCCACG252.8394297E-669.9847418
TCAACGA207.927418E-569.9847411
TGACTTG150.00222518369.9847412
TACCGCC150.00222518369.9847427
AGGGGGG176150.066.6275470
Produced by FastQC (version 0.11.5)