FastQCFastQC Report
Thu 26 May 2016
SRR1781484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179184
Sequences flagged as poor quality0
Sequence length76
%GC98

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16318291.06951513527993No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1950.10882668095365657No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1870.10436199660683988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA353.6579877E-970.076461
ATTAAAA353.6579877E-970.076462
TATCATT353.6579877E-970.076458
ATCATTA353.6579877E-970.076459
TCATTAA353.6579877E-970.076460
TTAAAAA353.6579877E-970.076463
GTATCAT401.3278623E-1070.056857
TGGCGGC207.8895544E-570.037219
GGGCTGC150.002217146870.037215
TCTGGCG252.822264E-670.0371911
CCGGCGG252.8316608E-669.9980421
GTGTGGA150.002227005369.9589236
GTAGATG150.00222947569.93938438
CTTATGC150.002231946769.919861
GTGCGCG150.002231946769.919864
GTCGCCG550.063.67018551
GCCGTAT550.063.67018554
GGTCGCC550.063.6523850
TAAAAAA501.4551915E-1163.06876464
CGTATCA501.4551915E-1163.05111756
Produced by FastQC (version 0.11.5)